El Hassania Loukili, Amal Elrherabi, Asmae Hbik, Amine Elbouzidi, Mohamed Taibi, Mohammed Merzouki, Mohamed Bouhrim, Abdelaaty A. Shahat, Omar M. Noman, Abdellah Azougay, Bruno Eto, Mohamed Bnouham, Belkheir Hammouti, Mohammed Ramdani. Chemical Analysis, Antihyperglycemic Properties and Enzyme Inhibition of Opuntia dillenii: A Detailed Analysis of Juice and Peel Extracts[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2025.101320
Citation:
El Hassania Loukili, Amal Elrherabi, Asmae Hbik, Amine Elbouzidi, Mohamed Taibi, Mohammed Merzouki, Mohamed Bouhrim, Abdelaaty A. Shahat, Omar M. Noman, Abdellah Azougay, Bruno Eto, Mohamed Bnouham, Belkheir Hammouti, Mohammed Ramdani. Chemical Analysis, Antihyperglycemic Properties and Enzyme Inhibition of Opuntia dillenii: A Detailed Analysis of Juice and Peel Extracts[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2025.101320
El Hassania Loukili, Amal Elrherabi, Asmae Hbik, Amine Elbouzidi, Mohamed Taibi, Mohammed Merzouki, Mohamed Bouhrim, Abdelaaty A. Shahat, Omar M. Noman, Abdellah Azougay, Bruno Eto, Mohamed Bnouham, Belkheir Hammouti, Mohammed Ramdani. Chemical Analysis, Antihyperglycemic Properties and Enzyme Inhibition of Opuntia dillenii: A Detailed Analysis of Juice and Peel Extracts[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2025.101320
Citation:
El Hassania Loukili, Amal Elrherabi, Asmae Hbik, Amine Elbouzidi, Mohamed Taibi, Mohammed Merzouki, Mohamed Bouhrim, Abdelaaty A. Shahat, Omar M. Noman, Abdellah Azougay, Bruno Eto, Mohamed Bnouham, Belkheir Hammouti, Mohammed Ramdani. Chemical Analysis, Antihyperglycemic Properties and Enzyme Inhibition of Opuntia dillenii: A Detailed Analysis of Juice and Peel Extracts[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2025.101320
1 Euromed University of Fes, UMEF, 30070, Fès, Morocco;
2. Laboratory of Applied Chemistry & Environment, Faculty of Sciences, University Mohammed 1st, Bd. Med VI B. P. 717, Oujda 60000, Morocco;
3 Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, University Mohammed 1st, Bd. Med VI B. P. 717, Oujda 60000, Morocco;
4 Laboratoire d'Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda 60000, Morocco;
5 Laboratory of Applied Chemistry Environment, Faculty of Science Oujda, University Mohammed First, Oujda 60000, Morocco;
6 Laboratories TBC, Laboratory of Pharmacology, Pharmacokinetics and Clinical Pharmacy, Faculty of Pharmacy, University of Lille, 59000 Lille, France;
7 Laboratory of Biological Engineering, Team of Functional and Pathological Biology, Faculty of Sciences and Technology, University Sultan Moulay Slimane, Beni Mellal 23000, Morocco;
8 Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 2457, Riyadh 11451, Saudi Arabia;
9 Laboratory of Applied Geosciences, Faculty of Sciences, Mohammed First University, Oujda 60000, Morocco
Funds:
We would like to extend our sincere appreciation to the Researchers Supporting Project number (RSPD2024R1057), King Saud University, Riyadh, Saudi Arabia.
Opuntia dillenii has been widely used in traditional medicine for various health conditions. This study examined the chemical composition of aqueous extracts from the plant's juice and peel and evaluated their effects on pancreatic α-amylase, lipase, and intestinal α-glucosidase enzymes, as well as their antihyperglycemic properties in vivo. Using High-Performance Liquid Chromatography with a Photodiode Array Detector (HPLC-DAD), significant variations in the composition were found between the plant's juice and peel, Key compounds included gallic acid, vanillic acid, p-coumaric acid, 3-hydroxy flavone, quercetin, cinnamic acid, kaempferol, and flavone. p-coumaric acid was highest in the juice (298.71±0.43 mg/100g) and peel (38.18±1.08 mg/100g), while flavone was higher in the peel (120.03±0.26 mg/100g). The extracts significantly inhibited pancreatic α-amylase and intestinal α-glucosidase in vitro, with confirmed in vivo effects reducing hyperglycemia in both healthy and diabetic rats. No toxicity was observed until the dose of 4000 mg/kg body weight. Molecular docking models showed that the plant's phytochemicals interacted with pancreatic enzymes more effectively than the drug acarbose. These findings highlight Opuntia dillenii's potential as a source of natural compounds with therapeutic properties, warranting further exploration.