Journal of Pharmaceutical Analysis

Analysis of isoquinoline alkaloids from Mahonia leschenaultia and Mahonia napaulensis roots using UHPLC-Orbitrap-MSn and UHPLC-QqQLIT-MS/MS

Awantika Singh, Vikas Bajpai, Sunil Kumar, Ajay Kumar Singh Rawat, Brijesh Kumar

2017, 7(2): 77-86.

Abstract(171) PDF(9)

Abstract:
Mahonia leschenaultia (ML) and Mahonia napaulensis (MN) are less known and unexplored medicinal plants of the family Berberidaceae. They are used by the Todas of Nilgiris in their religious and medical practices but chemically less identified. Hence, we decided to do extensive phytochemical analysis...

N-glycans released from glycoproteins using a commercial kit and comprehensively analyzed with a hypothetical database

Xue Sun, Lei Tao, Lin Yi, Yilan Ouyang, Naiyu Xu, Duxin Li, Robert J. Linhardt, Zhenqing Zhang

2017, 7(2): 87-94.

Abstract(138) PDF(6)

Abstract:
The glycosylation of proteins is responsible for their structural and functional roles in many cellular activities. This work describes a strategy that combines an efficient release, labeling and liquid chromatography–mass spectral analysis with the use of a comprehensive database to analyze N-glyca...

An improved LC–MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study

Dhiraj M. Rathod, Keyur R. Patel, Hiren N. Mistri, Arvind G. Jangid, Pranav S. Shrivastav, Mallika Sanyal

2017, 7(2): 95-102.

Abstract(119) PDF(5)

Abstract:
A highly sensitive and selective high performance liquid chromatography–tandem mass spectrometry method was developed and validated for the quantification of alverine (ALV) and its active metabolite, para hydroxy alverine (PHA), in human plasma. For sample preparation, solid phase extraction of anal...

Investigation of binding behaviour of procainamide hydrochloride with human serum albumin using synchronous, 3D fluorescence and circular dichroism

Kirthi Byadagi, Manjunath Meti, Sharanappa Nandibewoor, Shivamurti Chimatadar

2017, 7(2): 103-109.

Abstract(184) PDF(8)

Abstract:
Interaction of procainamide hydrochloride (PAH) with human serum albumin (HSA) is of great significance in understanding the pharmacokinetic and pharmacodynamic mechanisms of the drug. Multi-spectroscopic techniques were used to investigate the binding mode of PAH to HSA and results revealed the pre...

Square wave voltammetric quantification of folic acid, uric acid and ascorbic acid in biological matrix

Majid Arvand, Akram Pourhabib, Masoud Giahi

2017, 7(2): 110-117.

Abstract(141) PDF(7)

Abstract:
Nowadays, modified electrodes with metal nanoparticles have appeared as an alternative for the electroanalysis of various compounds. In this study, gold nanoparticles (GNPs) were chosen as interesting metal nanoparticles for modifying carbon paste electrode (CPE). GNPs and the gold nanoparticles-mod...

Degradation kinetics of larotaxel and identification of its degradation products in alkaline condition

Xiaoming Liang, Zhenzhen Liu, Huiyan Shi, Yuanyuan Zhang, Shixiao Wang, Kaishun Bi, Xiaohui Chen

2017, 7(2): 118-122.

Abstract(105) PDF(3)

Abstract:
Larotaxel, a new taxane compound prepared by partial synthesis from 10-deacetyl baccatin Ⅲ, is active against tumors. In this research, a selective LC–MS method was developed and validated for the study of degradation kinetics of larotaxel, which was carried out in aqueous solutions with different p...

Anti-atherosclerotic activity of root bark of Premna integrifolia Linn. in high fat diet induced atherosclerosis model rats

Chitra Subramani, Arivukkodi Rajakkannu, Arunadevi Rathinam, Sudesh Gaidhani, Ilavarasan Raju, Dhiman Vaidya Kartar Singh

2017, 7(2): 123-128.

Abstract(172) PDF(13)

Abstract:
Premna integrifolia Linn. is a medicinal plant used in"Dhasamula"drug preparation of Ayurvedic systems of medicine in the treatment of various ailments like bronchitis, dyspepsia, liver disorders, piles, constipation, hyperlipidemia and fever. The anti-atherosclerotic activity of hydroalcoholic extr...

A stability-indicating high performance liquid chromatography method to determine apocynin in nanoparticles

Juliana Kovalczuk de Oliveira, Débora Fernanda Veres Ronik, Jociani Ascari, Rubiana Mara Mainardes, Najeh Maissar Khalil

2017, 7(2): 129-133.

Abstract(211) PDF(3)

Abstract:
In this study, we developed and validated a fast, specific, sensitive, precise and stability-indicating high performance liquid chromatography (HPLC) method to determine the drug apocynin in bovine serum albumin (BSA) nanoparticles. Chromatographic analyses were performed on an RP C18 column and usi...

Development and validation of a stability-indicating RP–HPLC method for estimation of atazanavir sulfate in bulk

S. Dey, S. Subhasis Patro, N. Suresh Babu, P.N. Murthy, S.K. Panda

2017, 7(2): 134-140.

Abstract(225) PDF(9)

Abstract:
A stability-indicating reverse phase–high performance liquid chromatography (RP–HPLC) method was developed and validated for the determination of atazanavir sulfate in tablet dosage forms using C18 column Phenomenix (250 mm×4.6 mm, 5μm) with a mobile phase consisting of 900 mL of HPLC grade methanol...

2017 Vol. 7, No. 2