Jayesh A. Dhanani, Suzanne L. Parker, Jeffrey Lipman, Steven C. Wallis, Jeremy Cohen, John Fraser, Adrian Barnett, Michelle Chew, Jason A. Roberts. Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions[J]. Journal of Pharmaceutical Analysis, 2018, 8(6): 407-412.
Citation:
Jayesh A. Dhanani, Suzanne L. Parker, Jeffrey Lipman, Steven C. Wallis, Jeremy Cohen, John Fraser, Adrian Barnett, Michelle Chew, Jason A. Roberts. Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions[J]. Journal of Pharmaceutical Analysis, 2018, 8(6): 407-412.
Jayesh A. Dhanani, Suzanne L. Parker, Jeffrey Lipman, Steven C. Wallis, Jeremy Cohen, John Fraser, Adrian Barnett, Michelle Chew, Jason A. Roberts. Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions[J]. Journal of Pharmaceutical Analysis, 2018, 8(6): 407-412.
Citation:
Jayesh A. Dhanani, Suzanne L. Parker, Jeffrey Lipman, Steven C. Wallis, Jeremy Cohen, John Fraser, Adrian Barnett, Michelle Chew, Jason A. Roberts. Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions[J]. Journal of Pharmaceutical Analysis, 2018, 8(6): 407-412.
Burns, Trauma and Critical Care Research Centre, The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, QLD 4029, Australia
Department of Intensive Care Medicine, Royal Brisbane & Women's Hospital, Brisbane, Australia
Faculty of Health, Queensland University of Technology, Brisbane, Australia
Critical Care Research Group, The University of Queensland, Brisbane, Australia
Institute of Health and Biomedical Innovation & School of Public Health and Social Work, Queensland University of Technology, Kelvin Grove, Brisbane, Australia
Department of Anaesthesiology and Intensive Care, and Department of Medical and Health Sciences, Link?ping University, Link?ping, Sweden
School of Pharmacy, The University of Queensland, Brisbane, Australia
Department of Pharmacy, Royal Brisbane & Women's Hospital, Brisbane, Australia
Microdialysis is a technique used to measure the unbound antibiotic concentration in the interstitial spaces, the target site of action. In vitro recovery studies are essential to calibrating the microdialysis system for in vivo studies. The effect of a combination of antibiotics on recovery into microdialysate requires investigation. In vitro microdialysis recovery studies were conducted on a combination of vancomycin and tobramycin, in a simulated in vivo model. Comparison was made between recoveries for three different concentrations and three different perfusate flow rates. The overall relative recovery for vancomycinwas lower than that of tobramycin. For tobramycin, a concentration of 20μg/mL and flow rate of 1.0μL/min had the best recovery. A concentration of 5.0μg/mL and flow rate of 1.0μL/min yielded maximal recovery for vancomycin. Large molecular size and higher protein binding resulted in lower relative recoveries for vancomycin. Perfusate flow rates and drug concentrations affected the relative recovery when a combination of vancomycin and tobramycin was tested. Low perfusate flow rates were associated with higher recovery rates. For combination antibiotic measurement which includes agents that are highly protein bound, in vitro studies performed prior to in vivo studies may ensure the reliable measurement of unbound concentrations.
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Jayesh A. Dhanani, Suzanne L. Parker, Jeffrey Lipman, Steven C. Wallis, Jeremy Cohen, John Fraser, Adrian Barnett, Michelle Chew, Jason A. Roberts. Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions[J]. Journal of Pharmaceutical Analysis, 2018, 8(6): 407-412.
Jayesh A. Dhanani, Suzanne L. Parker, Jeffrey Lipman, Steven C. Wallis, Jeremy Cohen, John Fraser, Adrian Barnett, Michelle Chew, Jason A. Roberts. Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions[J]. Journal of Pharmaceutical Analysis, 2018, 8(6): 407-412.
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