Yue Liu, Taimei Cai, Sen Chen, Tao Wen, Hailong Peng. CeFe nanofibrous carbon nanozyme integrated with smartphone for the point-of-care testing of norfloxacin in water[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101023
Citation:
Yue Liu, Taimei Cai, Sen Chen, Tao Wen, Hailong Peng. CeFe nanofibrous carbon nanozyme integrated with smartphone for the point-of-care testing of norfloxacin in water[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101023
Yue Liu, Taimei Cai, Sen Chen, Tao Wen, Hailong Peng. CeFe nanofibrous carbon nanozyme integrated with smartphone for the point-of-care testing of norfloxacin in water[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101023
Citation:
Yue Liu, Taimei Cai, Sen Chen, Tao Wen, Hailong Peng. CeFe nanofibrous carbon nanozyme integrated with smartphone for the point-of-care testing of norfloxacin in water[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101023
1 Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province, School of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China;
2 School of Chemistry and Chemical Engineering, Nanchang University, Nanchang 330031, China;
3 College of Mechanical and Intelligent Manufacturing, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
The overuse of antibiotics has led to the severe contamination of water bodies, posing a considerable hazard to human health. Therefore, the development of an accurate and rapid point-of-care testing (POCT) platform for the quantitative detection of antibiotics is necessary. In this study, Cerium oxide (CeO2) and Ferrosoferric oxide (Fe3O4) nanoparticles were simultaneously encapsulated into N-doped nanofibrous carbon microspheres to form of a novel nanozyme (CeFe-NCMzyme) with a porous structure, high surface area, and N-doped carbon material properties, leading to a considerable enhancement of the peroxidase (POD)-like activity compared with that of the CeO2 or Fe3O4 nanoparticles alone. The POD-like activity of CeFe-NCMzyme can be quenched using L-Cysteine (Cys) and subsequently restored by the addition of a quinolone antibiotic (norfloxacin, NOR). Therefore, CeFe-NCMzyme was used as a colorimetric sensor to detect NOR via an “On-Off” model of POD-like activity. The sensor possessed a wide linear range of 0.05-20.0 μM (R2 = 0.9910) with a detection limit of 35.70 nM. Furthermore, a smartphone-assisted POCT platform with CeFe-NCMzyme was fabricated for quantitative detection of NOR based on RGB analysis. With the use of the POCT platform, a linear range of 0.1-20.0 μM and a detection limit of 54.10 nM were obtained. The spiked recoveries in the water samples were ranged from 97.73% to 102.01%, and the sensor exhibited good accuracy and acceptable reliability. This study provides a portable POCT platform for the on-site and quantitative monitoring of quinolone antibiotics in real samples, particularly in resource-constrained settings.