Qian Liu, Simin Zhang, Xiangyang Qu, Yunhui Xing, Zhenwei Xiao, Shicheng Fan, Janshon Zhu, Min Huang, Huichang Bi. A novel strategy based on the dielectric barrier discharge plasma for rapid eliminating the carryover associated with μPESI-MS/MS system[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101017
Citation:
Qian Liu, Simin Zhang, Xiangyang Qu, Yunhui Xing, Zhenwei Xiao, Shicheng Fan, Janshon Zhu, Min Huang, Huichang Bi. A novel strategy based on the dielectric barrier discharge plasma for rapid eliminating the carryover associated with μPESI-MS/MS system[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101017
Qian Liu, Simin Zhang, Xiangyang Qu, Yunhui Xing, Zhenwei Xiao, Shicheng Fan, Janshon Zhu, Min Huang, Huichang Bi. A novel strategy based on the dielectric barrier discharge plasma for rapid eliminating the carryover associated with μPESI-MS/MS system[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101017
Citation:
Qian Liu, Simin Zhang, Xiangyang Qu, Yunhui Xing, Zhenwei Xiao, Shicheng Fan, Janshon Zhu, Min Huang, Huichang Bi. A novel strategy based on the dielectric barrier discharge plasma for rapid eliminating the carryover associated with μPESI-MS/MS system[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101017
a. Guangdong Provincial Key Laboratory of New Drug Screening & NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Guangdong, China, 510515;
b. Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China, 510080;
c. Guangdong RangerBio Technologies Co., Ltd., Dongguan, Guangdong, China, 523000;
d. The State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University, Shenzhen, China 518055
Funds:
The authors would like to acknowledge the financial supports provided by the National Natural Science Foundation of China [grant number: 82025034 and 81973392], the Science and Technology Project of Guizhou province (project number: ZK [2022] General 410), the Social Development Technology Project of Dongguan (project number: 20211800907893) and the Shenzhen Science and Technology Program [No. KQTD20190929174023858].
In mass spectrometry (MS) analysis, carryover is a common and unavoidable problem. It causes over-estimation of analyte levels, which significantly influences accuracy and precision. Therefore, there are strict limits on carryover in MS-based assays. The conventional capillary-based electrospray ionization (ESI) restricts the use of MS for real-time monitoring, where a prompt response is crucial. Recently, we have developed a micro probe electrospray ionization (μPESI) coupled with MS (μPESI-MS) technology, enabling MS analysis within 40 seconds without a chromatographic column [1]. However, the challenge of carryover arises when trying to make the micro probe reusable for resource-saving purposes. Considering that dielectric barrier discharge (DBD) plasma is effective in degrading contaminants in water and gas [2], we hypothesized the eventual use of the DBD plasma to “wash” the micro probe. Then, a DBD plasma wash tube (DBD-WT) was introduced for rapid carryover elimination in μPESI-MS analysis.