Targeted bile acids metabolomics in cholesterol gallbladder polyps and gallstones: From analytical method development towards application to clinical samples

Jiaojiao Wei; Tao Chen; Yamin Liu; Shuai Sun; Zhiqing Yuan; Yixin Zhang; Aizhen Xiong; Linnan Li; Zhengtao Wang; Li Yang

doi:10.1016/j.jpha.2023.06.003

Volume 13 Issue 9

Sep. 2023

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Article Navigation > Journal of Pharmaceutical Analysis > 2023 > 13(9): 1080-1087

Jiaojiao Wei, Tao Chen, Yamin Liu, Shuai Sun, Zhiqing Yuan, Yixin Zhang, Aizhen Xiong, Linnan Li, Zhengtao Wang, Li Yang. Targeted bile acids metabolomics in cholesterol gallbladder polyps and gallstones: From analytical method development towards application to clinical samples[J]. Journal of Pharmaceutical Analysis, 2023, 13(9): 1080-1087. doi: 10.1016/j.jpha.2023.06.003

Citation:

Jiaojiao Wei, Tao Chen, Yamin Liu, Shuai Sun, Zhiqing Yuan, Yixin Zhang, Aizhen Xiong, Linnan Li, Zhengtao Wang, Li Yang. Targeted bile acids metabolomics in cholesterol gallbladder polyps and gallstones: From analytical method development towards application to clinical samples[J]. Journal of Pharmaceutical Analysis, 2023, 13(9): 1080-1087. doi: 10.1016/j.jpha.2023.06.003

Citation:

Jiaojiao Wei, Tao Chen, Yamin Liu, Shuai Sun, Zhiqing Yuan, Yixin Zhang, Aizhen Xiong, Linnan Li, Zhengtao Wang, Li Yang. Targeted bile acids metabolomics in cholesterol gallbladder polyps and gallstones: From analytical method development towards application to clinical samples[J]. Journal of Pharmaceutical Analysis, 2023, 13(9): 1080-1087. doi: 10.1016/j.jpha.2023.06.003

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Targeted bile acids metabolomics in cholesterol gallbladder polyps and gallstones: From analytical method development towards application to clinical samples

doi: 10.1016/j.jpha.2023.06.003

a. The MOE Key Laboratory of Standardization of Chinese Medicines, The SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines, The Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;
b. Department of Biliary and Pancreatic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China

Funds:

This work is financially supported by the National Natural Science Foundation of China (Grant Nos.: 81920108033, and 82274223).

Received Date: Mar. 14, 2023
Accepted Date: Jun. 05, 2023
Rev Recd Date: May 30, 2023
Publish Date: Jun. 07, 2023

Abstract

Abstract

Bile acids (BAs) are synthesized by the liver from cholesterol through several complementary pathways and aberrant cholesterol metabolism plays pivotal roles in the pathogeneses of cholesterol gallbladder polyps (CGP) and cholesterol gallstones (CGS). To date, there is neither systematic study on BAs profile of CGP or CGS, nor the relationship between them. To explore the metabolomics profile of plasma BAs in healthy volunteers, CGP and CGS patients, an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for simultaneous determination of 42 free and conjugated BAs in human plasma. The developed method was sensitive and reproducible to be applied for the quantification of BAs in the investigation of plasma samples. The results show that, compared to healthy volunteers, CGP and CGS were both characterized by the significant decrease in plasma BAs pool size, furthermore CGP and CGS shared aberrant BAs metabolic characteristics. Chenodeoxycholic acid, glycochenodeoxycholic acid, λ-muricholic acid, deoxycholic acid, and 7-ketolithocholic acid were shared potential markers of these two cholesterol gallbladder diseases. Subsequent analysis showed that clinical characteristics including cysteine, ornithine and body mass index might be closely related to metabolisms of certain BA modules. This work provides metabolomic information for the study of gallbladder diseases and analytical methodologies for clinical target analysis and efficacy evaluation related to BAs in medical institutions.
- Bile acid metabolism,
- Gallbladder polyps,
- Gallstones,
- Metabolomics,
- UPLC−MS/MS

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References(38)

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