Rapid authentication of different herbal medicines by heating online extraction electrospray ionization mass spectrometry

Zidong Qiu; Chaofa Wei; Xiang Li; Changjiangsheng Lai; Zhilai Zhan; Yan Jin; Li Zhou; Qingxiu Hao; Jian Yang; Shuanglong Wang; Liping Kang; Luqi Huang

doi:10.1016/j.jpha.2023.01.002

Volume 13 Issue 3

Mar. 2023

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Article Navigation > Journal of Pharmaceutical Analysis > 2023 > 13(3): 296-304

Zidong Qiu, Chaofa Wei, Xiang Li, Changjiangsheng Lai, Zhilai Zhan, Yan Jin, Li Zhou, Qingxiu Hao, Jian Yang, Shuanglong Wang, Liping Kang, Luqi Huang. Rapid authentication of different herbal medicines by heating online extraction electrospray ionization mass spectrometry[J]. Journal of Pharmaceutical Analysis, 2023, 13(3): 296-304. doi: 10.1016/j.jpha.2023.01.002

Citation:

Zidong Qiu, Chaofa Wei, Xiang Li, Changjiangsheng Lai, Zhilai Zhan, Yan Jin, Li Zhou, Qingxiu Hao, Jian Yang, Shuanglong Wang, Liping Kang, Luqi Huang. Rapid authentication of different herbal medicines by heating online extraction electrospray ionization mass spectrometry[J]. Journal of Pharmaceutical Analysis, 2023, 13(3): 296-304. doi: 10.1016/j.jpha.2023.01.002

Citation:

Zidong Qiu, Chaofa Wei, Xiang Li, Changjiangsheng Lai, Zhilai Zhan, Yan Jin, Li Zhou, Qingxiu Hao, Jian Yang, Shuanglong Wang, Liping Kang, Luqi Huang. Rapid authentication of different herbal medicines by heating online extraction electrospray ionization mass spectrometry[J]. Journal of Pharmaceutical Analysis, 2023, 13(3): 296-304. doi: 10.1016/j.jpha.2023.01.002

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Rapid authentication of different herbal medicines by heating online extraction electrospray ionization mass spectrometry

doi: 10.1016/j.jpha.2023.01.002

a. State Key Laboratory of Dao-di Herbs, National Resource Centre for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China;
b. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, College of Chemistry, Biology and Material Sciences, East China Institute of Technology, Nanchang, 330013, China

Funds:

This work was supported by the CACMS Innovation Fund, China (Grant Nos.: CI2021A04504 and CI2021A05206), the National Natural Science Foundation of China (Grant Nos.: 82104380, 81891010, 81891013, and 82074012), the Fundamental Research Funds for the Central Public Welfare Research Institutes, China (Grant Nos.: ZZ14-YQ-047 and ZZXT202105), the Key Project at Central Government Level (Grant No.: 2060302-2201-26), and the Beijing Nova Program.

Received Date: Jun. 11, 2022
Accepted Date: Jan. 31, 2023
Rev Recd Date: Jan. 18, 2023
Publish Date: Feb. 06, 2023

Abstract

Abstract

The rapid and accurate authentication of traditional Chinese medicines (TCMs) has always been a key scientific and technical problem in the field of pharmaceutical analysis. Herein, a novel heating online extraction electrospray ionization mass spectrometry (H-oEESI-MS) was developed for the rapid and direct analysis of extremely complex substances without the requirement for any sample pretreatment or pre-separation steps. The overall molecular profile and fragment structure features of various herbal medicines could be completely captured within 10–15 s, with minimal sample (<0.5 mg) and solvent consumption (<20 μL for one sample). Furthermore, a rapid differentiation and authentication strategy for TCMs based on H-oEESI-MS was proposed, including metabolic profile characterization, characteristic marker screening and identification, and multivariate statistical analysis model validation. In an analysis of 52 batches of seven types of Aconitum medicinal materials, 20 and 21 key compounds were screened out as the characteristic markers of raw and processed Aconitum herbal medicines, respectively, and the possible structures of all the characteristic markers were comprehensively identified based on Compound Discoverer databases. Finally, multivariate statistical analysis showed that all the different types of herbal medicines were well differentiated and identified (R²X > 0.87, R²Y > 0.91, and Q² > 0.72), which further verified the feasibility and reliability of this comprehensive strategy for the rapid authentication of different TCMs based on H-oEESI-MS. In summary, this rapid authentication strategy realized the ultra-high-throughput, low-cost, and standardized detection of various complex TCMs for the first time, thereby demonstrating wide applicability and value for the development of quality standards for TCMs.

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