Visualizing the spatial distribution and alteration of metabolites in continuously cropped <i>Salvia miltiorrhiza</i> Bge using MALDI-MSI

Chenglong Sun; Li Cui; Bingqian Zhou; Xiao Wang; Lanping Guo; Wei Liu

doi:10.1016/j.jpha.2021.09.011

Volume 12 Issue 5

Nov. 2022

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Chenglong Sun, Li Cui, Bingqian Zhou, Xiao Wang, Lanping Guo, Wei Liu. Visualizing the spatial distribution and alteration of metabolites in continuously cropped Salvia miltiorrhiza Bge using MALDI-MSI[J]. Journal of Pharmaceutical Analysis, 2022, 12(5): 719-724. doi: 10.1016/j.jpha.2021.09.011

Citation:

Chenglong Sun, Li Cui, Bingqian Zhou, Xiao Wang, Lanping Guo, Wei Liu. Visualizing the spatial distribution and alteration of metabolites in continuously cropped Salvia miltiorrhiza Bge using MALDI-MSI[J]. Journal of Pharmaceutical Analysis, 2022, 12(5): 719-724. doi: 10.1016/j.jpha.2021.09.011

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Visualizing the spatial distribution and alteration of metabolites in continuously cropped Salvia miltiorrhiza Bge using MALDI-MSI

doi: 10.1016/j.jpha.2021.09.011

Chenglong Sun^a,b,
Li Cui^a,b,
Bingqian Zhou^a,b,
Xiao Wang^a,b,
Lanping Guo^c,
Wei Liu^{a,b
,
,}

a. School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China;
b. Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China;
c. Resource Center of Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, 100700, China

Funds:

This work was supported by the Taishan Scholars Program of Shandong Province (Grant No.: tsqn202103096 for C. Sun and Grant No.: ts201511068 for L. Guo), the National Natural Science Foundation of China (Grant No.: 82003886), and the National Key R&

D Program of China (Grant Nos.: 2017YFC1700703 and 2017YFC1702701).

Received Date: May 04, 2021
Accepted Date: Sep. 17, 2021
Rev Recd Date: Aug. 30, 2021
Publish Date: Sep. 20, 2021

Abstract

Abstract

Salvia miltiorrhiza Bge (SMB) has long been used in traditional Chinese medicine to treat cardiovascular and cerebrovascular diseases. Growing clinical usage has led to a huge demand for artificial planting of SMB. Thus, continuous cropping of SMB is an important challenge that needs to be addressed. Continuous cropping can alter the metabolic profile of plants, resulting in poor growth and low yield. In this study, we tried to image the spatial location and variation of endogenous metabolites in continuously cropped SMB using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Spatially resolved expressions of tanshinones, salvianolic acids, polyamines, phenolic acids, amino acids, and oligosaccharides in normal and continuously cropped SMB roots were compared. The expressions of dihydrotanshinone I, tanshinone II A, dehydromiltirone, miltirone, dehydrotanshinone IIA, spermine, salvianolic acid B/E, tetrasaccharide, and pentasaccharide in continuously cropped SMB roots were much lower than those in normal roots. There was little difference in the expressions of caffeic acid and salvianolic acid A in normal and continuously cropped SMB roots. Ferulic acid was more widely distributed in xylem of normal SMB but strongly expressed in xylem, phloem, and cambium of continuously cropped SMB. The spatially resolved metabolite information enhances our understanding of the metabolic signature of continuously cropped SMB and also provides insights into the metabolic effects of continuous cropping in other plants.
- Metabolites,
- Spatial distribution and alteration,
- Continuous cropping,
- Salvia miltiorrhiza Bge,
- MALDI-MSI

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