An integrated strategy for comprehensive characterization of metabolites and metabolic profiles of bufadienolides from Venenum Bufonis in rats

Wen-Long Wei; Hao-Jv Li; Wen-Zhi Yang; Hua Qu; Zhen-Wei Li; Chang-Liang Yao; Jin-Jun Hou; Wan-Ying Wu; De-An Guo

doi:10.1016/j.jpha.2021.02.003

Volume 12 Issue 1

Feb. 2022

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Article Navigation > Journal of Pharmaceutical Analysis > 2022 > 12(1): 136-144

Wen-Long Wei, Hao-Jv Li, Wen-Zhi Yang, Hua Qu, Zhen-Wei Li, Chang-Liang Yao, Jin-Jun Hou, Wan-Ying Wu, De-An Guo. An integrated strategy for comprehensive characterization of metabolites and metabolic profiles of bufadienolides from Venenum Bufonis in rats[J]. Journal of Pharmaceutical Analysis, 2022, 12(1): 136-144. doi: 10.1016/j.jpha.2021.02.003

Citation:

Wen-Long Wei, Hao-Jv Li, Wen-Zhi Yang, Hua Qu, Zhen-Wei Li, Chang-Liang Yao, Jin-Jun Hou, Wan-Ying Wu, De-An Guo. An integrated strategy for comprehensive characterization of metabolites and metabolic profiles of bufadienolides from Venenum Bufonis in rats[J]. Journal of Pharmaceutical Analysis, 2022, 12(1): 136-144. doi: 10.1016/j.jpha.2021.02.003

Citation:

Wen-Long Wei, Hao-Jv Li, Wen-Zhi Yang, Hua Qu, Zhen-Wei Li, Chang-Liang Yao, Jin-Jun Hou, Wan-Ying Wu, De-An Guo. An integrated strategy for comprehensive characterization of metabolites and metabolic profiles of bufadienolides from Venenum Bufonis in rats[J]. Journal of Pharmaceutical Analysis, 2022, 12(1): 136-144. doi: 10.1016/j.jpha.2021.02.003

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An integrated strategy for comprehensive characterization of metabolites and metabolic profiles of bufadienolides from Venenum Bufonis in rats

doi: 10.1016/j.jpha.2021.02.003

a. Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China;
b. University of Chinese Academy of Sciences, Beijing, 100049, China

Funds:

This work was supported by the National Natural Science Foundation of China (Grant Nos.: 81530095 and 81673591), Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No.: XDA12020348), National Standardization of Traditional Chinese Medicine Project (Grant No.: ZYBZH-K-LN-01), and Science and Technology Commission Foundation of Shanghai (Grant No.: 15DZ0502800).

Received Date: Jun. 08, 2020
Accepted Date: Feb. 09, 2021
Rev Recd Date: Feb. 07, 2021
Publish Date: Feb. 12, 2021

Abstract

Abstract

Comprehensive characterization of metabolites and metabolic profiles in plasma has considerable significance in determining the efficacy and safety of traditional Chinese medicine (TCM) in vivo. However, this process is usually hindered by the insufficient characteristic fragments of metabolites, ubiquitous matrix interference, and complicated screening and identification procedures for metabolites. In this study, an effective strategy was established to systematically characterize the metabolites, deduce the metabolic pathways, and describe the metabolic profiles of bufadienolides isolated from Venenum Bufonis in vivo. The strategy was divided into five steps. First, the blank and test plasma samples were injected into an ultra-high performance liquid chromatography/linear trap quadrupole-orbitrap-mass spectrometry (MS) system in the full scan mode continuously five times to screen for valid matrix compounds and metabolites. Second, an extension-mass defect filter model was established to obtain the targeted precursor ions of the list of bufadienolide metabolites, which reduced approximately 39% of the interfering ions. Third, an acquisition model was developed and used to trigger more tandem MS (MS/MS) fragments of precursor ions based on the targeted ion list. The acquisition mode enhanced the acquisition capability by approximately four times than that of the regular data-dependent acquisition mode. Fourth, the acquired data were imported into Compound Discoverer software for identification of metabolites with metabolic network prediction. The main in vivo metabolic pathways of bufadienolides were elucidated. A total of 147 metabolites were characterized, and the main biotransformation reactions of bufadienolides were hydroxylation, dihydroxylation, and isomerization. Finally, the main prototype bufadienolides in plasma at different time points were determined using LC-MS/MS, and the metabolic profiles were clearly identified. This strategy could be widely used to elucidate the metabolic profiles of TCM preparations or Chinese patent medicines in vivo and provide critical data for rational drug use.
- Metabolic profiles,
- Extension-mass defect filter,
- Multidimensional data acquiring,
- Metabolic network prediction,
- Bufadienolides of Venenum Bufonis

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References(49)

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