Qualitative analysis of chemical components in Lianhua Qingwen capsule by HPLC-Q Exactive-Orbitrap-MS coupled with GC-MS

Shuai Fu; Rongrong Cheng; Zixin Deng; Tiangang Liu

doi:10.1016/j.jpha.2021.01.004

Volume 11 Issue 6

Dec. 2021

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Shuai Fu, Rongrong Cheng, Zixin Deng, Tiangang Liu. Qualitative analysis of chemical components in Lianhua Qingwen capsule by HPLC-Q Exactive-Orbitrap-MS coupled with GC-MS[J]. Journal of Pharmaceutical Analysis, 2021, 11(6): 709-716. doi: 10.1016/j.jpha.2021.01.004

Citation:

Shuai Fu, Rongrong Cheng, Zixin Deng, Tiangang Liu. Qualitative analysis of chemical components in Lianhua Qingwen capsule by HPLC-Q Exactive-Orbitrap-MS coupled with GC-MS[J]. Journal of Pharmaceutical Analysis, 2021, 11(6): 709-716. doi: 10.1016/j.jpha.2021.01.004

Citation:

Shuai Fu, Rongrong Cheng, Zixin Deng, Tiangang Liu. Qualitative analysis of chemical components in Lianhua Qingwen capsule by HPLC-Q Exactive-Orbitrap-MS coupled with GC-MS[J]. Journal of Pharmaceutical Analysis, 2021, 11(6): 709-716. doi: 10.1016/j.jpha.2021.01.004

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Qualitative analysis of chemical components in Lianhua Qingwen capsule by HPLC-Q Exactive-Orbitrap-MS coupled with GC-MS

doi: 10.1016/j.jpha.2021.01.004

Shuai Fu^{a
,
,},
Rongrong Cheng^a,
Zixin Deng^a,b,
Tiangang Liu^{a
,
,}

a. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071, China;
b. State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200030, China

Funds:

This work is financially supported by the Fundamental Research Funds for the Central Universities (Grant No.: 2042020kf1003).

Received Date: Jun. 02, 2020
Accepted Date: Jan. 25, 2021
Rev Recd Date: Jan. 07, 2021
Available Online: Jan. 12, 2022
Publish Date: Dec. 15, 2021

Abstract

Abstract

The Lianhua Qingwen (LHQW) capsule is a popular traditional Chinese medicine for the treatment of viral respiratory diseases. In particular, it has been recently prescribed to treat infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, due to its complex composition, little attention has been directed toward the analysis of chemical constituents present in the LHQW capsule. This study presents a reliable and comprehensive approach to characterizing the chemical constituents present in LHQW by high-performance liquid chromatography-Q Exactive-Orbitrap mass spectrometry (HPLC-Q Exactive-Orbitrap-MS) coupled with gas chromatography-mass spectrometry (GC-MS). An automated library alignment method with a high mass accuracy (within 5 ppm) was used for the rapid identification of compounds. A total of 104 compounds, consisting of alkaloids, flavonoids, phenols, phenolic acids, phenylpropanoids, quinones, terpenoids, and other phytochemicals, were successfully characterized. In addition, the fragmentation pathways and characteristic fragments of some representative compounds were elucidated. GC-MS analysis was conducted to characterize the volatile compounds present in LHQW. In total, 17 compounds were putatively characterized by comparing the acquired data with that from the NIST library. The major constituent was menthol, and all the other compounds were terpenoids. This is the first comprehensive report on the identification of the major chemical constituents present in the LHQW capsule by HPLC-Q Exactive-Orbitrap-MS, coupled with GC-MS, and the results of this study can be used for the quality control and standardization of LHQW capsules.
- Lianhua Qingwen capsule,
- HPLC-Q exactive-orbitrap-MS,
- GC-MS,
- Chemical components

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References(49)

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