A DNA-based nanocarrier for efficient cancer therapy

Muhammad Abbas; Mirza Muhammad Faran Ashraf Baig; Yaliang Zhang; Yu-Shun Yang; Songyu Wu; Yiqiao Hu; Zhong-Chang Wang; Hai-Liang Zhu

doi:10.1016/j.jpha.2020.03.005

Volume 11 Issue 3

Jun. 2021

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Article Navigation > Journal of Pharmaceutical Analysis > 2021 > 11(3): 330-339

Muhammad Abbas, Mirza Muhammad Faran Ashraf Baig, Yaliang Zhang, Yu-Shun Yang, Songyu Wu, Yiqiao Hu, Zhong-Chang Wang, Hai-Liang Zhu. A DNA-based nanocarrier for efficient cancer therapy[J]. Journal of Pharmaceutical Analysis, 2021, 11(3): 330-339. doi: 10.1016/j.jpha.2020.03.005

Citation:

Muhammad Abbas, Mirza Muhammad Faran Ashraf Baig, Yaliang Zhang, Yu-Shun Yang, Songyu Wu, Yiqiao Hu, Zhong-Chang Wang, Hai-Liang Zhu. A DNA-based nanocarrier for efficient cancer therapy[J]. Journal of Pharmaceutical Analysis, 2021, 11(3): 330-339. doi: 10.1016/j.jpha.2020.03.005

Citation:

Muhammad Abbas, Mirza Muhammad Faran Ashraf Baig, Yaliang Zhang, Yu-Shun Yang, Songyu Wu, Yiqiao Hu, Zhong-Chang Wang, Hai-Liang Zhu. A DNA-based nanocarrier for efficient cancer therapy[J]. Journal of Pharmaceutical Analysis, 2021, 11(3): 330-339. doi: 10.1016/j.jpha.2020.03.005

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A DNA-based nanocarrier for efficient cancer therapy

doi: 10.1016/j.jpha.2020.03.005

a. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210023, PR China;
b. State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, PR China;
c. Institute of Drug Research and Development, Medical School of Nanjing University, Nanjing, 210093, PR China

Funds:

This work was supported by the China Scholarship Council (CSC) grant (Grant No. 20180500458). We also thank the platform for characterization and test at State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, China for the assistance with the instrumentation.

Received Date: Nov. 25, 2019
Accepted Date: Mar. 05, 2020
Rev Recd Date: Mar. 02, 2020
Available Online: Jan. 24, 2022
Publish Date: Jun. 15, 2021

Abstract

Abstract

The study aimed to achieve enhanced targeted cytotoxicity and cell-internalization of cisplatin-loaded deoxyribonucleic acid-nanothread (CPT-DNA-NT), mediated by scavenger receptors into HeLa cells. DNA-NT was developed with stiff-topology utilizing circular-scaffold to encapsulate CPT. Atomic force microscopy (AFM) characterization of the DNA-NT showed uniformity in the structure with a diameter of 50–150 nm and length of 300–600 nm. The successful fabrication of the DNA-NT was confirmed through native-polyacrylamide gel electrophoresis analysis, as large the molecular-weight (polymeric) DNA-NT did not split into constituting strands under applied current and voltage. The results of cell viability confirmed that blank DNA-NT had the least cytotoxicity at the highest concentration (512 nM) with a viability of 92% as evidence of its biocompatibility for drug delivery. MTT assay showed superior cytotoxicity of CPT-DNA-NT than that of the free CPT due to the depot release of CPT after DNA-NT internalization. The DNA-NT exhibited targeted cell internalizations with the controlled intracellular release of CPT (from DNA-NT), as illustrated in confocal images. Therefore, in vitro cytotoxicity assessment through flow cytometry showed enhanced apoptosis (72.7%) with CPT-DNA-NT (compared to free CPT; 64.4%). CPT-DNA-NT, being poly-anionic, showed enhanced endocytosis via scavenger receptors.
- DNA-nanothread,
- Cisplatin,
- Drug delivery,
- Scavenger receptors,
- Caveolae,
- HeLa cell line

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References(44)

References

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