Analytical quality-by-design approach for sample treatment of BSA-containing solutions

Lien Taevernier; Evelien Wynendaele; Matthias D’Hondt; Bart De Spiegeleer

doi:10.1016/j.jpha.2014.06.001

Volume 5 Issue 1

Feb. 2015

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Lien Taevernier, Evelien Wynendaele, Matthias D’Hondt, Bart De Spiegeleer. Analytical quality-by-design approach for sample treatment of BSA-containing solutions[J]. Journal of Pharmaceutical Analysis, 2015, (1): 27-32. doi: 10.1016/j.jpha.2014.06.001

Citation:

Lien Taevernier, Evelien Wynendaele, Matthias D’Hondt, Bart De Spiegeleer. Analytical quality-by-design approach for sample treatment of BSA-containing solutions[J]. Journal of Pharmaceutical Analysis, 2015, (1): 27-32. doi: 10.1016/j.jpha.2014.06.001

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Analytical quality-by-design approach for sample treatment of BSA-containing solutions

doi: 10.1016/j.jpha.2014.06.001

Drug Quality and Registration DruQuaR Group, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium

Funds:

The authors would like to thank the Special Research Fund of Ghent University

the Institute for the Promotion of Innovation through Science and Technology in Flanders

Publish Date: Feb. 10, 2015

Abstract

Abstract

The sample preparation of samples containing bovine serum albumin (BSA), e.g., as used in transdermal Franz diffusion cell (FDC) solutions, was evaluated using an analytical quality-by-design (QbD) approach. Traditional precipitation of BSA by adding an equal volume of organic solvent, often successfully used with conventional HPLC-PDA, was found insufficiently robust when novel fused-core HPLC and/or UPLC-MS methods were used. In this study, three factors (acetonitrile (%), formic acid (%) and boiling time (min)) were included in the experimental design to determine an optimal and more suitable sample treatment of BSA-containing FDC solutions. Using a QbD and Derringer desirability (D) approach, combining BSA loss, dilution factor and variability, we constructed an optimal working space with the edge of failure defined as Do0.9. The design space is modelled and is confirmed to have an ACN range of 8373%and FA content of 170.25%.