Synchronized separation of atorvastatin-an antihyperlipidemic drug with antihypertensive, antidiabetic,antithrombotic drugs by RP-LC for determination in combined formulations

A new rapid and sensitive high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination of atorvastatin-an antihyperlipidemic drug along with most commonly prescribed drugs (antihyperlipidemic,antihypertensive,antidiabetic,antithrombotic) in bulk and marketed combined formulations.The chromatographic separation was carried out by gradient elution mode with acetonitrile as organic modifier and 0.1% triethylamine acetate (TEAA) buffer pH 5 at a flow rate of 1 mL/min and a diode array detector at wavelength 230 nm was employed for detection of the analytcs.Calibration curves were linear in the range of 5 -150 μg/mL for all the drugs with correlation coefficients of determination (r2 values) ≥ 0.999.Limits of detection (LODs) and Limits of quantification (LOQs) ranged from 0.1 to 0.27 μg/mL and 0.3 to 0.89 μg/mL respectively.Intra-day and inter-day precision was studied at three concentration levels (20,60 and 100 μg/mL).The intra-day and inter-day RSD for all compounds was less than 2.0%.The accuracy for all compounds was found to be between 98% and 102%.Thus,the performance of the method described allows its use in quantification of atorvastatin along with 9 most commonly prescribed drugs available in market as atorvastatin combined dosage forms.