Journal of Pharmaceutical Analysis

Stability study on an anti-cancer drug 4-(3,5-bis(2-chlorobenzylidene)-4-oxo-piperidine-1-yl)-4-oxo-2-butenoic acid (CLEFMA) using a stability-indicating HPLC method

Dhawal Raghuvanshi, Gregory Nkepang, Alamdar Hussain, Hooman Yari, Vibhudutta Awasthi?

2017, 7(1): 1-9.

Abstract(184) PDF(1) [Cited by] (14)

Abstract:
CLEFMA, 4-(3,5-bis(2-chlorobenzylidene)-4-oxo-piperidine-1-yl)-4-oxo-2-butenoic acid, is a new chemical entity with anti-cancer and anti-inflammatory activities. Here, we report its stability in solution against stress conditions of exposure to acid/base, light, oxidant, high temperature, and plasma...

Bioinformatic prediction and functional characterization of human KIAA0100 gene

He Cui, Xi Lan, Shemin Lu, Fujun Zhang, Wanggang Zhang

2017, 7(1): 10-18.

Abstract(129) PDF(1) [Cited by] (7)

Abstract:
Our previous study demonstrated that human KIAA0100 gene is a novel acute monocytic leukemia-associated antigen (MLAA) gene. But the functional characterization of human KIAA0100 gene has remained unknown to date. Here, firstly, bioinformatic prediction of human KIAA0100 gene was carried out using o...

Binding interaction of phosphorus heterocycles with bovine serum albumin:A biochemical study

Swarup Roy, Raj Kumar Nandi, Sintu Ganai, K.C. Majumdar, Tapan K. Das

2017, 7(1): 19-26.

Abstract(119) PDF(6) [Cited by] (90)

Abstract:
Interaction between bovine serum albumin (BSA) and phosphorus heterocycles (PHs) was studied using multi-spectroscopic techniques. The results indicated the high binding a?nity of PHs to BSA as it quenches the intrinsic fluorescence of BSA. The experimental data suggested the fluorescence quenching ...

Fabrication of an electrochemical sensor for determination of doxorubicin in human plasma and its interaction with DNA

Reza Hajian, Zahra Tayebi, Nafiseh Shams

2017, 7(1): 27-33.

Abstract(173) PDF(10) [Cited by] (92)

Abstract:
In this work, an electrochemical sensor was fabricated for determination of an anthracycline, doxorubicin (DOX) as a chemotherapy drug in plasma based on multi-walled carbon nanotubes modified platinum electrode (Pt/MWCNTs). DOX was effectively accumulated on the surface of modified electrode and ge...

N-alkylamide profiling of Achillea ptarmica and Achillea millefolium extracts by liquid and gas chromatography-mass spectrometry

Lieselotte Veryser, Lien Taevernier, Evelien Wynendaele, Yannick Verheust, Ann Dumoulin, Bart De Spiegeleer

2017, 7(1): 34-47.

Abstract(142) PDF(3) [Cited by] (19)

Abstract:
Achillea millefolium and Achillea ptarmica are both plants belonging to the Asteracea family and are traditionally used for their medicinal properties. It has already been shown that some N-alkylamides (NAAs) are responsible for these pharmacological actions. Therefore, in the present study, the NAA...

Analysis of bacitracin and its related substances by liquid chromatography tandem mass spectrometry

Suleiman Ahmed Suleiman, Fan Song, Mengxiang Su, Taijun Hang, Min Song

2017, 7(1): 48-55.

Abstract(250) PDF(10) [Cited by] (19)

Abstract:
A suitable liquid chromatography quadrupole time-of-flight mass spectrometric (LC–Q-TOF–MS) method was developed for separation and characterization of related substances in bacitracin test drug. The separation was performed on LiChrospher RP-18 column using methanol as mobile phase A and 0.2% ammon...

Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography-tandem mass spectrometry method in human plasma

Dhiraj M. Rathod, Keyur R. Patel, Hiren N. Mistri, Arvind G. Jangid, Pranav S. Shrivastav, Mallika Sanyal

2017, 7(1): 56-62.

Abstract(89) PDF(1)

Abstract:
The present study describes a simple, reliable and reproducible liquid chromatography–tandem mass spectro-metry method (LC–MS/MS) for the simultaneous determination of allopurinol and its active metabolite, oxypurinol in human plasma for a pharmacokinetic/bioequivalence study. After protein precipit...

Determination of fenticonazole in human plasma by HPLC-MS/MS and its application to pharmacokinetic studies

Weixing Mao, Yiya Wang, Wenhui Hu, Feifei Jiao, Hongwei Fan, Li Ding

2017, 7(1): 63-70.

Abstract(149) PDF(0)

Abstract:
Two simple and sensitive high performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) methods were developed and validated for the determination of fenticonazole in human plasma after percutaneous and intravaginal administration. Mifepristone was used as an internal standard (IS), an...

Bile salt induced solubilization of methylene blue:Study on methylene blue fluorescence properties and molecular mechanics calculation

Susithra Selvam, Ivy Sarkar

2017, 7(1): 71-75.

Abstract(118) PDF(2) [Cited by] (54)

Abstract:
Methylene blue (MB) is a hydrophobic drug molecule, having importance both as a staining reagent and pharmaceutical agent. MB is strongly fluorescent, with an emission peak at 686 nm (λex 665 nm). In the study, the possibility of MB as an extrinsic fluorophore to study the micellization behavior of ...

2017 Vol. 7, No. 1