Isolation of Blood-Derived Extracellular Vesicles for Disease Diagnosis: Methods, Challenges, and Applications

Xi Song; Jacques Crommen; Marianne Fillet

doi:10.1016/j.jpha.2026.101640

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Xi Song, Jacques Crommen, Marianne Fillet. Isolation of Blood-Derived Extracellular Vesicles for Disease Diagnosis: Methods, Challenges, and Applications[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2026.101640

Citation:

Xi Song, Jacques Crommen, Marianne Fillet. Isolation of Blood-Derived Extracellular Vesicles for Disease Diagnosis: Methods, Challenges, and Applications[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2026.101640

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Isolation of Blood-Derived Extracellular Vesicles for Disease Diagnosis: Methods, Challenges, and Applications

doi: 10.1016/j.jpha.2026.101640

Laboratory for the Analysis of Medicines (LAM), Department of Pharmacy, CIRM, University of Liege, Avenue Hippocrate,15, 4000 Liège, Belgium

Funds:

This work was supported by grants the Fonds de la Recherche Scientifique (FNRS, Grant No.: 40008414).

Received Date: Oct. 20, 2025
Accepted Date: Apr. 16, 2026
Rev Recd Date: Apr. 16, 2026
Available Online: Apr. 18, 2026

Abstract

Abstract

Extracellular vesicles (EVs) are a family of cell-derived membrane vesicles found in almost all body fluids. Blood-derived EVs are particularly attractive for clinical diagnostics due to their accessibility and systemic representation. However, the intrinsic complexity of EVs and the abundance of non-EV particles in blood have hindered the establishment of a standardized isolation method. Meanwhile, research on EV isolation is rapidly evolving, and there is a clear need for reviews that synthesize the recent advances in this field, particularly those focusing on blood-derived EVs. In this review, we provide a comprehensive overview of both established and emerging isolation techniques, summarizing their advantages, limitations, and applications reported over the past years, to provide methodological insights and guidance for future isolation studies.
- extracellular vesicles,
- blood,
- proteomics,
- biomarkers

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References(114)

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