Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement

Zhiqiang Zhang; Li Wang; Xia Ma; Hui Wang

doi:10.1016/j.jpha.2023.12.012

Volume 14 Issue 12

Dec. 2024

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Zhiqiang Zhang, Li Wang, Xia Ma, Hui Wang. Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement[J]. Journal of Pharmaceutical Analysis, 2024, 14(12): 100922. doi: 10.1016/j.jpha.2023.12.012

Citation:

Zhiqiang Zhang, Li Wang, Xia Ma, Hui Wang. Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement[J]. Journal of Pharmaceutical Analysis, 2024, 14(12): 100922. doi: 10.1016/j.jpha.2023.12.012

Citation:

Zhiqiang Zhang, Li Wang, Xia Ma, Hui Wang. Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement[J]. Journal of Pharmaceutical Analysis, 2024, 14(12): 100922. doi: 10.1016/j.jpha.2023.12.012

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Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement

doi: 10.1016/j.jpha.2023.12.012

Zhiqiang Zhang^a,
Li Wang^b,
Xia Ma^{c
,
,},
Hui Wang^{a
,
,}

a. College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450045, China;
b. Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou, 450046, China;
c. College of Animal Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China

Received Date: Sep. 08, 2023
Accepted Date: Dec. 16, 2023
Rev Recd Date: Dec. 14, 2023
Publish Date: Dec. 22, 2023

Abstract

Abstract

A series of biodegradable nanoparticle-based drug delivery systems have been designed utilizing poly(β-amino ester)-guanidine-phenylboronic acid (PBAE-G) polymers. In this study, a novel Lentinan-Functionalized PBAE-G-nanodiamond system was developed to carry ovalbumin (LNT-PBAE-G-ND@OVA). The impact of this drug delivery system on the activation and maturation of macrophages was then assessed. Furthermore, LNT-PBAE-G-ND@OVA induced potent antibody response and showed no obvious toxicity in vitro and in vivo. Moreover, treatment with LNT-PBAE-G-ND@OVA was sufficient to alter the expression of genes associated with the cGAS-STING pathway, and the LNT-PBAE-G-ND@OVA induced upregulation of costimulatory molecules. LNT-PBAE-G-ND@OVA treatment was sufficient to induce macrophage activation through a complex mechanism in which cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) synthase (cGAS)-stimulator of interferon genes (STING) signaling plays an integral role.
- LNT-PBAE-G-ND@OVA,
- Molecular mechanisms,
- cGAS-STING signaling

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References(41)

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