Volume 7 Issue 1
Feb.  2017
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Dhiraj M. Rathod, Keyur R. Patel, Hiren N. Mistri, Arvind G. Jangid, Pranav S. Shrivastav, Mallika Sanyal. Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography-tandem mass spectrometry method in human plasma[J]. Journal of Pharmaceutical Analysis, 2017, 7(1): 56-62.
Citation: Dhiraj M. Rathod, Keyur R. Patel, Hiren N. Mistri, Arvind G. Jangid, Pranav S. Shrivastav, Mallika Sanyal. Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography-tandem mass spectrometry method in human plasma[J]. Journal of Pharmaceutical Analysis, 2017, 7(1): 56-62.

Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography-tandem mass spectrometry method in human plasma

  • Publish Date: Feb. 10, 2017
  • The present study describes a simple, reliable and reproducible liquid chromatography–tandem mass spectro-metry method (LC–MS/MS) for the simultaneous determination of allopurinol and its active metabolite, oxypurinol in human plasma for a pharmacokinetic/bioequivalence study. After protein precipitation (PPT) of 100 μL plasma sample with 1.0%formic acid in acetonitrile, the recovery of the analytes and allopurinol-d2 as an internal standard ranged from 85.36% to 91.20%. The analytes were separated on Hypersil Gold (150 mm×4.6 mm, 5 μm) column using 0.1% formic acid-acetonitrile (98:2, v/v) as the mobile phase. Quantification was done using electrospray ionization in the positive mode. The calibration concentration range was established from 60.0 to 6000 ng/mL for allopurinol and 80.0–8000 ng/mL for oxypurinol. Matrix effect in human plasma, expressed as IS-normalized matrix factors ranged from 1.003 to 1.030 for both the analytes. The developed method was found suitable for a clinical study with 300 mg allopurinol tablet formulation in healthy subjects.
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