Bing-Bing Shang, Jun Sha, Yang Liu, Qin Tu, Man-Lin Li, Jin-Yi Wang. Synthesis of a new chitosan derivative and assay of Escherichia coli adsorption[J]. Journal of Pharmaceutical Analysis, 2011, 01(1): 39-45.
Citation:
Bing-Bing Shang, Jun Sha, Yang Liu, Qin Tu, Man-Lin Li, Jin-Yi Wang. Synthesis of a new chitosan derivative and assay of Escherichia coli adsorption[J]. Journal of Pharmaceutical Analysis, 2011, 01(1): 39-45.
Bing-Bing Shang, Jun Sha, Yang Liu, Qin Tu, Man-Lin Li, Jin-Yi Wang. Synthesis of a new chitosan derivative and assay of Escherichia coli adsorption[J]. Journal of Pharmaceutical Analysis, 2011, 01(1): 39-45.
Citation:
Bing-Bing Shang, Jun Sha, Yang Liu, Qin Tu, Man-Lin Li, Jin-Yi Wang. Synthesis of a new chitosan derivative and assay of Escherichia coli adsorption[J]. Journal of Pharmaceutical Analysis, 2011, 01(1): 39-45.
College of Science, Northwest Agricultural & Forestry University, Yangling, Shaanxi 712100, China
Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest Agricultural & Forestry University, Yangling, Shaanxi 712100, China.
Funds:
the Scientific Research Foundation for the Returned Overseas Chinese Scholars of the Ministry of Education,and Northwest Agricultural & Forestry University
the Ministry of Education of the People's Republic of China
the State Forestry Administration of the People's Republic of China
A new chitosan derivative is prepared using chitosan.Ethyl chlorocarbonate was first introduced to the hydroxyl group of phthaloylchitosan through a nucleophilic reaction.Hydrazine was then added to recover the amino groups of chitosan and promote cross-linking.The structure of this new chitosan derivative was characterized by Fourier transform infrared (FT-IR) and proton nuclear magnetic resonance (1H NMR) spectroscopy,and its physical properties were determined by X-ray diffraction (XRD),differential scanning calorimetry (DSC),and thermogravimetric analysis (TGA).The thermal and chemical stabilities of the new derivative were improved compared with those of native chitosan.Assay of Escherichia coli adhesion on a film based on this chitosan derivative showed good adsorption and biofilm formation.