Small-molecule probes based on natural products: Elucidation of drug-target mechanisms in stroke

Xingyue Jin; Suyi Liu; Shujing Chen; Rui Han; Xingyi Sun; Mingyan Wei; Yanxu Chang; Lin Li; Han Zhang

doi:10.1016/j.jpha.2025.101290

Volume 15 Issue 11

Nov. 2025

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Xingyue Jin, Suyi Liu, Shujing Chen, Rui Han, Xingyi Sun, Mingyan Wei, Yanxu Chang, Lin Li, Han Zhang. Small-molecule probes based on natural products: Elucidation of drug-target mechanisms in stroke[J]. Journal of Pharmaceutical Analysis, 2025, 15(11): 101290. doi: 10.1016/j.jpha.2025.101290

Citation:

Xingyue Jin, Suyi Liu, Shujing Chen, Rui Han, Xingyi Sun, Mingyan Wei, Yanxu Chang, Lin Li, Han Zhang. Small-molecule probes based on natural products: Elucidation of drug-target mechanisms in stroke[J]. Journal of Pharmaceutical Analysis, 2025, 15(11): 101290. doi: 10.1016/j.jpha.2025.101290

Citation:

Xingyue Jin, Suyi Liu, Shujing Chen, Rui Han, Xingyi Sun, Mingyan Wei, Yanxu Chang, Lin Li, Han Zhang. Small-molecule probes based on natural products: Elucidation of drug-target mechanisms in stroke[J]. Journal of Pharmaceutical Analysis, 2025, 15(11): 101290. doi: 10.1016/j.jpha.2025.101290

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Small-molecule probes based on natural products: Elucidation of drug-target mechanisms in stroke

doi: 10.1016/j.jpha.2025.101290

Xingyue Jin^a,b,c,
Suyi Liu^a,c,
Shujing Chen^a,c,
Rui Han^a,b,c,
Xingyi Sun^a,b,c,
Mingyan Wei^a,b,c,
Yanxu Chang^{a,b,c
,
,},
Lin Li^{a,b,c
,
,},
Han Zhang^{a,b,c
,
,}

a. State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China;
b. Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, Tianjin, 301617, China;
c. State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China

Funds:

This work was supported by the Tianjin Science and Technology Plan Project (Project No.: 23ZYJDSS00020), the National Natural Science Foundation of China (Grant No.: 82004331), and the Key Research and Development Program of HuBei Province of China (Program No.: 2022ACA003-02-002). Figures were drawn using BioGDP (https://biogdp.com/).

Received Date: Dec. 03, 2024
Accepted Date: Mar. 28, 2025
Rev Recd Date: Mar. 13, 2025
Publish Date: Apr. 03, 2025

Abstract

Abstract

Natural products (NPs) are an important source of new drugs for the treatment of stroke. Identifying cellular targets for bioactive molecules is a major challenge and critical issue in the development of new drugs for stroke. Small-molecule probes play a unique role in target discovery. However, drawbacks to these probes include non-specificity, unstable activity, and difficulty in synthesis. Small-molecule probes based on NPs at least partially compensate for these shortcomings. NPs feature rich chemical and structural diversity, biocompatibility, and unique biological activities. These features could be exploited to provide new ideas and tools for target discovery. Small-molecule probes based on NPs provide a precise and direct search for interacting protein targets of NPs-active small molecules. This review explores the properties of small-molecule probes based on NPs and their applications in mechanistic studies of stroke and other diseases. We hope that this review will bring new perspectives to the mechanistic study of NPs-active small molecules and accelerate the translation of these ingredients into drug candidates for the treatment of stroke.
- Small-molecule probes,
- Natural products,
- Protein identification,
- Stroke,
- Labeled probes,
- Unlabeled probes

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References(133)

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