Investigation of oligomeric proanthocyanidins extracted from Rhodiolae Crenulatae Radix et Rhizomes using deep eutectic solvents and identified via data-dependent acquisition mass-spectroscopy

Li Jia; Liming Wang; Xiaoxiao Zhang; Qingrui Zhang; Peng Lei; Yanxu Chang; Lifeng Han; Xin Chai; Wenzhi Yang; Yuefei Wang; Miaomiao Jiang

doi:10.1016/j.jpha.2024.101002

Volume 14 Issue 11

Nov. 2024

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Article Navigation > Journal of Pharmaceutical Analysis > 2024 > 14(11): 101002

Li Jia, Liming Wang, Xiaoxiao Zhang, Qingrui Zhang, Peng Lei, Yanxu Chang, Lifeng Han, Xin Chai, Wenzhi Yang, Yuefei Wang, Miaomiao Jiang. Investigation of oligomeric proanthocyanidins extracted from Rhodiolae Crenulatae Radix et Rhizomes using deep eutectic solvents and identified via data-dependent acquisition mass-spectroscopy[J]. Journal of Pharmaceutical Analysis, 2024, 14(11): 101002. doi: 10.1016/j.jpha.2024.101002

Citation:

Li Jia, Liming Wang, Xiaoxiao Zhang, Qingrui Zhang, Peng Lei, Yanxu Chang, Lifeng Han, Xin Chai, Wenzhi Yang, Yuefei Wang, Miaomiao Jiang. Investigation of oligomeric proanthocyanidins extracted from Rhodiolae Crenulatae Radix et Rhizomes using deep eutectic solvents and identified via data-dependent acquisition mass-spectroscopy[J]. Journal of Pharmaceutical Analysis, 2024, 14(11): 101002. doi: 10.1016/j.jpha.2024.101002

Citation:

Li Jia, Liming Wang, Xiaoxiao Zhang, Qingrui Zhang, Peng Lei, Yanxu Chang, Lifeng Han, Xin Chai, Wenzhi Yang, Yuefei Wang, Miaomiao Jiang. Investigation of oligomeric proanthocyanidins extracted from Rhodiolae Crenulatae Radix et Rhizomes using deep eutectic solvents and identified via data-dependent acquisition mass-spectroscopy[J]. Journal of Pharmaceutical Analysis, 2024, 14(11): 101002. doi: 10.1016/j.jpha.2024.101002

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Investigation of oligomeric proanthocyanidins extracted from Rhodiolae Crenulatae Radix et Rhizomes using deep eutectic solvents and identified via data-dependent acquisition mass-spectroscopy

doi: 10.1016/j.jpha.2024.101002

a. National Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China;
b. Haihe Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China

Funds:

This work was supported by the Science and Technology Program of Tianjin in China (Grant No.: 23ZYJDSS00030) and the Science and Technology Project of Haihe Laboratory of Modern Chinese Medicine, China (Grant Nos.: 22HHZYSS00007 and 22HHZYJC00003).

Received Date: Jul. 23, 2023
Accepted Date: May 09, 2024
Rev Recd Date: May 05, 2024
Publish Date: May 11, 2024

Abstract

Abstract

In this study, 34 deep eutectic solvents (DESs) were successfully prepared for the extraction of proanthocyanidin from Rhodiolae Crenulatae Radix et Rhizomes. The extraction process was optimized using single factor exploration and Box-Behnken design-response surface analysis. The extraction rate was significantly improved when the molar ratio of choline chloride to 1,3-propanediol was 1:3.5 and the water content was 30% (V/V) in DESs. AB-8 macroporous resin and ethyl acetate were used for separation and refining, and the oligomer-rich proanthocyanidin components were eventually obtained. The ultraviolet (UV) and infrared (IR) spectra showed that the proanthocyanidins were mainly composed of catechin and epicatechin. To further clarify the chemical composition of proanthocyanidin, an ion scan list containing 156 proanthocyanidins precursors was obtained by constructing a proanthocyanidins structural library and mass defect filtering (MDF) algorithm, combined with the full mass spectrometry (MS)/dd-MS² scan mode that turns on the “if idle pick others” function. By using ultra-high performance liquid chromatography and high-resolution MS (UHPLC/HRMS), the analysis used both targeted and non-targeted methods to detect proanthocyanidins. Finally, 50 oligomeric proanthocyanidin (OPC) compounds were identified, including 7 monomers, 22 dimers, 20 trimers, and 1 tetramer, most of which were procyanidins of proanthocyanidins (84%), and a small amount of prodelphinidin (14%) and other types of proanthocyanidins (2%), which enabled the systematic characterization of proanthocyanidin components from Rhodiolae Crenulatae Radix et Rhizomes. Meanwhile, the comparison with the grape seeds OPCs standard (United States Pharmacopeia) revealed that the proanthocyanidins in Rhodiolae Crenulatae Radix et Rhizomes were more abundant, suggesting that the proanthocyanidins in Rhodiolae Crenulatae Radix et Rhizomes has promising applications.
- Proanthocyanidins,
- Deep eutectic solvent,
- Rhodiolae Crenulatae Radix et Rhizomes,
- Pharmacodynamic material basis

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References(45)

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