Quantitative estimation of enzymatic released specific oligosaccharides from <i>Hericium erinaceus</i> polysaccharides using CE-LIF

Yong Deng; Jing Zhao; Shaoping Li

doi:10.1016/j.jpha.2022.11.004

Volume 13 Issue 2

Mar. 2023

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Article Navigation > Journal of Pharmaceutical Analysis > 2023 > 13(2): 201-208

Yong Deng, Jing Zhao, Shaoping Li. Quantitative estimation of enzymatic released specific oligosaccharides from Hericium erinaceus polysaccharides using CE-LIF[J]. Journal of Pharmaceutical Analysis, 2023, 13(2): 201-208. doi: 10.1016/j.jpha.2022.11.004

Citation:

Yong Deng, Jing Zhao, Shaoping Li. Quantitative estimation of enzymatic released specific oligosaccharides from Hericium erinaceus polysaccharides using CE-LIF[J]. Journal of Pharmaceutical Analysis, 2023, 13(2): 201-208. doi: 10.1016/j.jpha.2022.11.004

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Quantitative estimation of enzymatic released specific oligosaccharides from Hericium erinaceus polysaccharides using CE-LIF

doi: 10.1016/j.jpha.2022.11.004

Yong Deng^a,b,c,
Jing Zhao^{a,b,c
,
,},
Shaoping Li^{a,b,c
,
,}

a. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, 999078, China;
b. Joint Laboratory of Chinese Herbal Glycoengineering and Testing Technology, University of Macau, Macao SAR, 999078, China;
c. Macao Centre for Testing of Chineese Medicine, University of Macau, Macao SAR, 999078, China

Funds:

The research was partially funded by grants from the National Natural Science Foundation of China (Grant No.: 81673389), the Science and Technology Development Fund, Macao SAR, China (File Nos.: 0075/2018/A2, 034/2017/A1 and 0017/2019/AKP), the Guangdong Key Project for Modernization of Lingnan Herbs (Project No.: 2020B1111110006), and the Multi-Year Research Grant from the University of Macau (File Nos.: MYRG2018-00083-ICMS, MYRG2019-00128-ICMS, and CPG2022-00014-ICMS).

Received Date: Jul. 25, 2022
Accepted Date: Nov. 10, 2022
Rev Recd Date: Nov. 08, 2022
Publish Date: Mar. 07, 2023

Abstract

Abstract

Polysaccharides exhibit multiple pharmacological activities which are closely related to their structural features. Therefore, quantitatively quality control of polysaccharides based on their chemical characteristics is important for their application in biomedical and functional food sciences. However, polysaccharides are mixed macromolecular compounds that are difficult to isolate and lack standards, making them challenging to quantify directly. In this study, we proposed an improved saccharide mapping method based on the release of specific oligosaccharides for the assessment of Hericium erinaceus polysaccharides from laboratory cultured and different regions of China. Briefly, a polysaccharide from H. erinaceus was digested by β-(1-3)-glucanase, and the released specific oligosaccharides were labeled with 8-aminopyrene-1,3,6-trisulfonic-acid (APTS) and separated by using micellar electrokinetic chromatography (MEKC) coupled with laser induced fluorescence (LIF), and quantitatively estimated. MEKC presented higher resolution compared to polysaccharide analysis using carbohydrate gel electrophoresis (PACE), and provided great peak capacity between oligosaccharides with polymerization degree of 2 (DP2) and polymerization degree of 6 (DP6) in a dextran ladder separation. The results of high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RI) showed that 12 h was sufficient for complete digestion of polysaccharides from H. erinaceus. Laminaritriose (DP3) was used as an internal standard for quantification of all the oligosaccharides. The calibration curve for DP3 showed a good linear regression (R² > 0.9988). The limit of detection (LOD) and limit of quantification (LOQ) values were 0.05 μg/mL and 0.2 μg/mL, respectively. The recovery for DP3 was 87.32 (±0.03)% in the three independent injections. To sum up, this proposed method is helpful for improving the quality control of polysaccharides from H. erinaceus as well as other materials.
- Saccharide mapping,
- Hericium erinaceus,
- Polysaccharide,
- CE-LIF,
- PACE

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References

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