Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells

Xin-Yuan Hu; Jia-Jing Li; Zi-Wei Yang; Jun Zhang; Huai-Song Wang

doi:10.1016/j.jpha.2022.05.007

Volume 12 Issue 5

Nov. 2022

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Xin-Yuan Hu, Jia-Jing Li, Zi-Wei Yang, Jun Zhang, Huai-Song Wang. Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells[J]. Journal of Pharmaceutical Analysis, 2022, 12(5): 801-807. doi: 10.1016/j.jpha.2022.05.007

Citation:

Xin-Yuan Hu, Jia-Jing Li, Zi-Wei Yang, Jun Zhang, Huai-Song Wang. Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells[J]. Journal of Pharmaceutical Analysis, 2022, 12(5): 801-807. doi: 10.1016/j.jpha.2022.05.007

Citation:

Xin-Yuan Hu, Jia-Jing Li, Zi-Wei Yang, Jun Zhang, Huai-Song Wang. Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells[J]. Journal of Pharmaceutical Analysis, 2022, 12(5): 801-807. doi: 10.1016/j.jpha.2022.05.007

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Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells

doi: 10.1016/j.jpha.2022.05.007

a. Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China;
b. College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin, 300392, China

Funds:

This work was supported by the National Natural Science Foundation of China (Grant No.: 21705165), the Open Project Program of the MOE Key Laboratory of Drug Quality Control and Pharmacovigilance (Grant No.: DQCP20/21MS03), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and "Double First-Class" University Project (Grant No.: CPU2018GF07).

Received Date: Nov. 22, 2021
Accepted Date: May 22, 2022
Rev Recd Date: May 12, 2022
Publish Date: May 28, 2022

Abstract

Abstract

The catalytic generation of H₂ in living cells provides a method for antioxidant therapy. In this study, an [FeFe]-hydrogenase mimic [Ru + Fe₂S₂@F127(80)] was synthesized by self-assembling polymeric pluronic F-127, catalytic [Fe₂S₂] sites, and photosensitizer Ru(bpy)₃²⁺. Under blue light irradiation, hydrated protons were photochemically reduced to H₂, which increased the local pH in living cells (HeLa cells). The generated H₂ was subsequently used as an antioxidant to decrease reactive oxygen species (ROS) levels in living cells (HEK 293T, HepG2, MCF-7, and HeLa cells). Our findings revealed that the proliferation of HEK 293T cells increased by a factor of about six times, relative to that of other cells (HepG2, MCF-7, and HeLa cells). Intracellular ROS and pH levels were then monitored using fluorescent cell imaging. Our study showed that cell imaging can be used to evaluate the ability of Ru + Fe₂S₂@F127 to eliminate oxidative stress and prevent ROS-related diseases.
- Cell imaging,
- [FeFe]-hydrogenase,
- Reactive oxygen species,
- Photocatalytic system

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References(38)

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