Volume 12 Issue 4
Sep.  2022
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Lingyuan Xu, Xiuyuan Zhang, A.M. Abd El-Aty, Yuanshang Wang, Zhen Cao, Huiyan Jia, J.-Pablo Salvador, Ahmet Hacimuftuoglu, Xueyan Cui, Yudan Zhang, Kun Wang, Yongxin She, Fen Jin, Lufei Zheng, Baima Pujia, Jing Wang, Maojun Jin, Bruce D. Hammock. A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching[J]. Journal of Pharmaceutical Analysis, 2022, 12(4): 637-644. doi: 10.1016/j.jpha.2022.05.004
Citation: Lingyuan Xu, Xiuyuan Zhang, A.M. Abd El-Aty, Yuanshang Wang, Zhen Cao, Huiyan Jia, J.-Pablo Salvador, Ahmet Hacimuftuoglu, Xueyan Cui, Yudan Zhang, Kun Wang, Yongxin She, Fen Jin, Lufei Zheng, Baima Pujia, Jing Wang, Maojun Jin, Bruce D. Hammock. A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching[J]. Journal of Pharmaceutical Analysis, 2022, 12(4): 637-644. doi: 10.1016/j.jpha.2022.05.004

A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence anti-quenching

doi: 10.1016/j.jpha.2022.05.004
Funds:

This work was supported by the Central Public Interest Scientific Institution Basal Research Fund for the Chinese Academy of Agricultural Sciences (Grant No.: Y2021PT05), National Institute of Environmental Health Science Superfund Research Program (Grant No.: P42 ES004699), National Academy of Sciences (Subaward No.: 2000009144), and Ningbo Innovation Project for Agro-Products Quality and Safety (Grant No.: 2019CXGC007).

  • Received Date: Oct. 12, 2021
  • Accepted Date: May 14, 2022
  • Rev Recd Date: May 11, 2022
  • Publish Date: May 20, 2022
  • Balancing the risks and benefits of organophosphate pesticides (OPs) on human and environmental health relies partly on their accurate measurement. A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs (triazophos, parathion, and chlorpyrifos) in apples, turnips, cabbages, and rice. Gold nanoparticles were functionalized with monoclonal antibodies against the tested OPs. DNA oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal amplification. The detection signals were generated by DNA-RNA hybridization and ribonuclease H dissociation of the fluorophore. The resulting fluorescence signal enables multiplexed quantification of triazophos, parathion, and chlorpyrifos residues over the concentration range of 0.01-25, 0.01-50, and 0.1-50 ng/mL with limits of detection of 0.014, 0.011, and 0.126 ng/mL, respectively. The mean recovery ranged between 80.3% and 110.8% with relative standard deviations of 7.3%-17.6%, which correlate well with results obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The proposed bio-barcode immunoassay is stable, reproducible and reliable, and is able to detect low residual levels of multi-residue OPs in agricultural products.
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  • M. Hasanuzzaman, M.A. Rahman, M.S. Islam, et al., Pesticide residues analysis in water samples of Nagarpur and Saturia Upazila, Bangladesh. Applied Water Sci. 8 (2018) 1-6
    J. Butler-Dawson, K. Galvin, P.S. Thorne, et al., Organophosphorus pesticide exposure and neurobehavioral performance in Latino children living in an orchard community, Neurotoxicology 53 (2016) 165-172
    G. Briceño, H. Schalchli, A. Mutis, et al., Use of pure and mixed culture of diazinon-degrading Streptomyces to remove other organophosphorus pesticides, Int. Biodeterior. Biodegrad. 114 (2016) 193-201
    X. Zou, X. Xiao, H. Zhou, et al., Effects of soil acidification on the toxicity of organophosphorus pesticide on Eisenia fetida and its mechanism, J. Hazard. Mater. 539 (2018) 365-372
    M. Li, X. Hua, M. Ma, et al., Detecting clothianidin residues in environmental and agricultural samples using rapid, sensitive enzyme-linked immunosorbent assay and gold immunochromatographic assay, Sci. Total Environ. 499 (2014) 1-6
    S. Wang, L. Ge, L. Li, et al., Molecularly imprinted polymer grafted paper-based multi-disk micro-disk plate for chemiluminescence detection of pesticide, Biosens. Bioelectron. 50 (2013) 262-268
    J.M. Hicks, Fluorescence immunoassay. Hum. Pathol. 15 (1984) 112-116
    A. Khanmohammadi, A.J. Ghazizadeh, P. Hashemi, et al., An overview to electrochemical biosensors and sensors for the detection of environmental contaminants, J. Iran. Chem. Soc. 17 (2020) 2429-2447
    S.M. Taha, S.A. Gadalla, Development of an efficient method for multi residue analysis of 160 pesticides in herbal plant by ethyl acetate hexane mixture with direct injection to GC-MS/MS, Talanta 174 (2017) 767-779
    Y. Wang, M. Jin, G. Chen, et al., Bio-barcode detection technology and its research applications:A review, J. Adv. Res. 20 (2019) 23-32
    S.I. Stoeva, J.S. Lee, C.S. Thaxton, et al., Multiplexed DNA detection with biobarcoded nanoparticle probes, Angew. Chem. Int. Ed. Engl. 45 (2006) 3303-3306
    Y. Li, Y.T. Cu, D. Luo, Multiplexed detection of pathogen DNA with DNA-based fluorescence nanobarcodes, Nat. Biotechnol. 23 (2005) 885-889
    X. Lin, Y. Liu, Z. Tao, et al., Nanozyme-based bio-barcode assay for high sensitive and logic-controlled specific detection of multiple DNAs, Biosens. Bioelectron. 94 (2017) 471-477
    S. Lv, K. Zhang, Z. Z. Lin, et al., Novel photoelectrochemical immunosensor for disease-related protein assisted by hemin/G-quadruplex-based DNAzyme on gold nanoparticles to enhance cathodic photocurrent on p-CuBi2O4 semiconductor, Biosens. Bioelectron. 96 (2017) 317-323
    Y. Lin, Q. Zhou, D. Tang, et al., Signal-on photoelectrochemical immunoassay for aflatoxin B1 based on enzymatic product-etching MnO2 nanosheets for dissociation of carbon dots, Anal. Chem. 89 (2017) 5637-5645
    H. Dong, X. Meng, W. Dai, et al., Highly sensitive and selective microRNA detection based on DNA-bio-bar-code and enzyme-assisted strand cycle exponential signal amplification, Anal. Chem. 87 (2015) 4334-4340
    J. Chen, Z. Jiang, J.D. Ackerman, et al., Electrochemical nanoparticle-enzyme sensors for screening bacterial contamination in drinking water, Analyst 140 (2015) 4991-4996
    J. Gao, X. Huang, H. Liu, et al., Colloidal stability of gold nanoparticles modified with thiol compounds:bioconjugation and application in cancer cell imaging, Langmuir 28 (2012) 4464-4471
    T. Hu, S. Lu, C. Chen, et al., Colorimetric sandwich immunosensor for Aβ(1-42) based on dual antibody-modified gold nanoparticles, Sens. Actuat. B. 243 (2017) 792-799
    C.-Y Lee, R.-J. Shiau, H.-W. Chou, et al., Combining aptamer-modified gold nanoparticles with barcode DNA sequence amplification for indirect analysis of ethanolamine, Sens. Actuat. B. 254 (2018) 189-196
    S. Parween, A. Ali, V.S. Chauhan, Non-natural amino acids containing peptide-capped gold nanoparticles for drug delivery application, ACS Appl. Mater. Interfaces 5 (2013) 6484-6493
    Y. Liu, Y. Liu, M. Zhou, et al., Chemiluminescence detection of protein in capillary electrophoresis using aptamer-functionalized gold nanoparticles as biosensing platform, J. Chromatogr. A 1340 (2014) 128-133
    Y. Liang, Z. Zhang, Z. Liu, et al., A highly sensitive signal-amplified gold nanoparticle-based electrochemical immunosensor for dibutyl phthalate detection, Biosens. Bioelectron. 91 (2017) 199-202
    J.M. Nam, A.R. Wise, J.T. Groves, Colorimetric bio-barcode amplification assay for cytokines, Anal. Chem. 77 (2005) 6985-6988
    J. Liu, Z. Guan, Z. Lv, et al., Improving sensitivity of gold nanoparticle based fluorescence quenching and colorimetric aptasensor by using water resuspended gold nanoparticle, Biosens. Bioelectron. 52 (2014) 265-270
    G. Liu, X. Yang, T. Li, et al., Spectrophotometric and visual detection of the herbicide atrazine by exploiting hydrogen bond-induced aggregation of melamine-modified gold nanoparticles, Microchimica Acta 182 (2015) 1983-1989
    G. Yang, H. Zhuang, H. Chen, et al., A gold nanoparticle based immunosorbent bio-barcode assay combined with real-time immuno-PCR for the detection of polychlorinated biphenyls, Sens. Actuat. B. Chem. 214 (2015) 152-158
    L. Du, W. Ji, Y. Zhang, et al., An ultrasensitive detection of 17β-estradiol using a gold nanoparticle-based fluorescence immunoassay, Analyst 140 (2015) 2001-2007
    D. Lin, J. Wu, F. Yan, et al., Ultrasensitive immunoassay of protein biomarker based on electrochemiluminescent quenching of quantum dots by hemin bio-bar-coded nanoparticle tags, Anal. Chem. 83 (2011) 5214-5221
    C. Zhang, Z. Jiang, M. Jin, et al., Fluorescence immunoassay for multiplex detection of organophosphate pesticides in agro-products based on signal amplification of gold nanoparticles and oligonucleotides, Food Chem. 326 (2020), 126813
    X. Zhang, P. Du, X. Cui, et al., A sensitive fluorometric bio-barcodes immunoassay for detection of triazophos residue in agricultural products and water samples by iterative cycles of dna-rna hybridization and dissociation of fluorophores by ribonucleaseh, The Sci. Total Environ. 717 (2020), 137268
    G. Chen, M. Jin, J. Ma, et al. Competitive bio-barcode immunoassay for highly sensitive detection of parathion based on bimetallic nanozyme catalysis, J. Agric. Food. Chem. 68 (2020) 660-668
    C. Zhang, P. Du, Z. Jiang, et al., A simple and sensitive competitive bio-barcode immunoassay for triazophos based on multi-modified gold nanoparticles and fluorescent signal amplification, Anal. Chim. Acta 999 (2018) 123-131
    R. Zou, Y. Chang, T. Zhang, et al., Up-converting nanoparticle-based immunochromatographic strip for multi-residue detection of three organophosphorus pesticides in food, Front Chem. 7 (2019), 18
    M. Jin, H. Shao, F. Jin, et al., Enhanced competitive chemiluminescent enzyme immunoassay for the trace detection of insecticide triazophos, J. Food Sci. 77 (2012) T99-T104
    Q. Liu, J. Tian, M. Jiang, et al., Direct competitive biomimetic immunoassay based on quantum dot label for simultaneous determination of two pesticide residues in fruit and vegetable samples, Food Anal. Methods 11 (2018) 3015-3022
    Z. Xu, Q. Wang, H. Lei, et al., A simple, rapid and high-throughput fluorescence polarization immunoassay for simultaneous detection of organophosphorus pesticides in vegetable and environmental water samples, Anal. Chimica Acta 708 (2011) 123-129
    Y. Li, F. Zhao, L. Zhao, et al., Development of a broad-specificity immunoassay for determination of organophosphorus pesticides using dual-generic hapten antigens, Food Anal. Methods 8 (2015) 420-427
    Y. Guo, J. Tian, C. Liang, et al., Multiplex bead-array competitive immunoassay for simultaneous detection of three pesticides in vegetables, Microchimica Acta 180 (2013) 387-395
    R. Zou, Y. Liu, S. Wang, et al., Development and evaluation of chemiluminescence enzyme-linked immunoassay for residue detection of three organophosphorus pesticides, Chin. J. Pesticide Sci. 19 (2017) 37-45
    P. Du, M. Jin, G. Chen, et al., A competitive bio-barcode amplification immunoassay for small molecules based on nanoparticles, Sci. Rep. 6 (2016), 38114
    P. Du, M. Jin, G. Chen, et al., Competitive colorimetric triazophos immunoassay employing magnetic microspheres and multi-labeled gold nanoparticles along with enzymatic signal enhancement, Microchimica Acta 184 (2017) 3705-3712
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