Cyclic arginine-glycine-aspartic acid-modified red blood cells for drug delivery: Synthesis and <i>in vitro</i> evaluation

Chen Wang; Min Wang; Yan Zhang; Hongxin Jia; Binbin Chen

doi:10.1016/j.jpha.2021.06.003

Volume 12 Issue 2

May 2022

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Article Navigation > Journal of Pharmaceutical Analysis > 2022 > 12(2): 324-331

Chen Wang, Min Wang, Yan Zhang, Hongxin Jia, Binbin Chen. Cyclic arginine-glycine-aspartic acid-modified red blood cells for drug delivery: Synthesis and in vitro evaluation[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 324-331. doi: 10.1016/j.jpha.2021.06.003

Citation:

Chen Wang, Min Wang, Yan Zhang, Hongxin Jia, Binbin Chen. Cyclic arginine-glycine-aspartic acid-modified red blood cells for drug delivery: Synthesis and in vitro evaluation[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 324-331. doi: 10.1016/j.jpha.2021.06.003

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Cyclic arginine-glycine-aspartic acid-modified red blood cells for drug delivery: Synthesis and in vitro evaluation

doi: 10.1016/j.jpha.2021.06.003

a. Department of Pharmaceutics, School of Pharmacy, Xiamen Medical College, Xiamen, Fujian, 361023, China;
b. Institute of Respiratory Diseases, Xiamen Medical College, Xiamen, Fujian, 361023, China;
c. Department of Pharmaceutics, School of Pharmacy, Harbin University of Commerce, Harbin, 150076, China;
d. Department of Pharmacy, Xiamen Xianyue Hospital, Xiamen, Fujian, 361012, China

Funds:

The authors are grateful for the financial support provided by the General Program of the Natural Science Foundation of Fujian Province of China (Grant No.: 2019D016), the Program of the Institute of Respiratory Diseases of Xiamen Medical College (Program No.: HXJB-04), the New Century Excellent Talent Support Program of Higher Education Institutions of Fujian Province (Program No.: MinJiaoKe [2018] 47), and the Innovation and Entrepreneurship Training Program for College Students (Program No.: 201912631017).

Received Date: Sep. 22, 2020
Accepted Date: Jun. 07, 2021
Rev Recd Date: Jun. 05, 2021
Publish Date: Jun. 12, 2021

Abstract

Abstract

Red blood cells (RBCs) are an excellent choice for cell preparation research because of their biocompatibility, high drug loading, and long half-life. In this study, doxorubicin (DOX) was encapsulated with RBCs as the carrier. The biotin-avidin system binding principle was used to modify biotinylated cyclic arginine-glycine-aspartic acid (cRGD) onto RBC surfaces for accurate targeting, high drug loading, and sustained drug release. The RBC drug delivery system (DDS) was characterized, and the concentration of surface sulfur in the energy spectrum was 6.330%. The physical and chemical properties of RBC DDS were as follows: drug content, 0.857 mg/mL; particle size, 3339 nm; potential value, -12.5 mV; and cumulative release rate, 81.35%. There was no significant change in RBC morphology for up to seven days. The results of the targeting and cytotoxicity studies of RBC DDS showed that many RBCs covered the surfaces of U251 cells, and the fluorescence intensity was higher than that of MCF-7 cells. The IC₅₀ value of unmodified drug-loaded RBCs was 2.5 times higher than that of targeted modified drug-loaded RBCs, indicating that the targeting of cancer cells produced satisfactory inhibition. This study confirms that the RBC DDS has the characteristics of accurate targeting, high drug loading, and slow drug release, which increases its likelihood of becoming a clinical cancer treatment in the future.
- Cytotoxicity,
- Red blood cells,
- Drug delivery,
- Targeting

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References(47)

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