A review on the screening methods for the discovery of natural antimicrobial peptides

Bin Yang; Hongyan Yang; Jianlong Liang; Jiarou Chen; Chunhua Wang; Yuanyuan Wang; Jincai Wang; Wenhui Luo; Tao Deng; Jialiang Guo

doi:10.1016/j.jpha.2024.101046

Volume 15 Issue 1

Feb. 2025

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Article Navigation > Journal of Pharmaceutical Analysis > 2025 > 15(1): 101046

Bin Yang, Hongyan Yang, Jianlong Liang, Jiarou Chen, Chunhua Wang, Yuanyuan Wang, Jincai Wang, Wenhui Luo, Tao Deng, Jialiang Guo. A review on the screening methods for the discovery of natural antimicrobial peptides[J]. Journal of Pharmaceutical Analysis, 2025, 15(1): 101046. doi: 10.1016/j.jpha.2024.101046

Citation:

Bin Yang, Hongyan Yang, Jianlong Liang, Jiarou Chen, Chunhua Wang, Yuanyuan Wang, Jincai Wang, Wenhui Luo, Tao Deng, Jialiang Guo. A review on the screening methods for the discovery of natural antimicrobial peptides[J]. Journal of Pharmaceutical Analysis, 2025, 15(1): 101046. doi: 10.1016/j.jpha.2024.101046

Citation:

Bin Yang, Hongyan Yang, Jianlong Liang, Jiarou Chen, Chunhua Wang, Yuanyuan Wang, Jincai Wang, Wenhui Luo, Tao Deng, Jialiang Guo. A review on the screening methods for the discovery of natural antimicrobial peptides[J]. Journal of Pharmaceutical Analysis, 2025, 15(1): 101046. doi: 10.1016/j.jpha.2024.101046

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A review on the screening methods for the discovery of natural antimicrobial peptides

doi: 10.1016/j.jpha.2024.101046

a. School of Medicine, Foshan University, Foshan, Guangdong, 528000, China;
b. College of Pharmacy, Jinan University, Guangzhou, 510632, China;
c. Guangdong Yifang Pharmaceutical Co., Ltd., Foshan, Guangdong, 528244, China

Funds:

This work was supported by the National Natural Science Foundation of China (Grant Nos.: 82373835, 82304437, and 82173781), Regional Joint Fund Project of Guangdong Basic and Applied Basic Research Fund, China (Grant Nos.: 2023A1515110417 and 2023A1515140131), Regional Joint Fund-Key Project of Guangdong Basic and Applied Basic Research Fund, China (Grant No.: 2020B1515120033), the Key Field Projects of General Universities in Guangdong Province, China (Grant Nos.: 2020ZDZX2057 and 2022ZDZX2056), and Medical Scientific Research Foundation of Guangdong Province of China (Grant No.: A2022061).

Received Date: Apr. 02, 2024
Accepted Date: Jul. 16, 2024
Rev Recd Date: Jul. 08, 2024
Publish Date: Jul. 18, 2024

Abstract

Abstract

Natural antimicrobial peptides (AMPs) are promising candidates for the development of a new generation of antimicrobials to combat antibiotic-resistant pathogens. They have found extensive applications in the fields of medicine, food, and agriculture. However, efficiently screening AMPs from natural sources poses several challenges, including low efficiency and high antibiotic resistance. This review focuses on the action mechanisms of AMPs, both through membrane and non-membrane routes. We thoroughly examine various highly efficient AMP screening methods, including whole-bacterial adsorption binding, cell membrane chromatography (CMC), phospholipid membrane chromatography binding, membrane-mediated capillary electrophoresis (CE), colorimetric assays, thin layer chromatography (TLC), fluorescence-based screening, genetic sequencing-based analysis, computational mining of AMP databases, and virtual screening methods. Additionally, we discuss potential developmental applications for enhancing the efficiency of AMP discovery. This review provides a comprehensive framework for identifying AMPs within complex natural product systems.
- Antimicrobial peptides,
- Natural products,
- High-throughput screening,
- Mechanism

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References(141)

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