Ultrasensitive quantification of trace amines based on <i>N</i>-phosphorylation labeling chip 2D LC-QQQ/MS

Xiqing Bian; Yida Zhang; Na Li; Menglin Shi; Xiaolin Chen; Hui-Lu Zhang; Jie Liu; Jian-Lin Wu

doi:10.1016/j.jpha.2023.02.003

Volume 13 Issue 3

Mar. 2023

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Article Navigation > Journal of Pharmaceutical Analysis > 2023 > 13(3): 315-322

Xiqing Bian, Yida Zhang, Na Li, Menglin Shi, Xiaolin Chen, Hui-Lu Zhang, Jie Liu, Jian-Lin Wu. Ultrasensitive quantification of trace amines based on N-phosphorylation labeling chip 2D LC-QQQ/MS[J]. Journal of Pharmaceutical Analysis, 2023, 13(3): 315-322. doi: 10.1016/j.jpha.2023.02.003

Citation:

Xiqing Bian, Yida Zhang, Na Li, Menglin Shi, Xiaolin Chen, Hui-Lu Zhang, Jie Liu, Jian-Lin Wu. Ultrasensitive quantification of trace amines based on N-phosphorylation labeling chip 2D LC-QQQ/MS[J]. Journal of Pharmaceutical Analysis, 2023, 13(3): 315-322. doi: 10.1016/j.jpha.2023.02.003

Citation:

Xiqing Bian, Yida Zhang, Na Li, Menglin Shi, Xiaolin Chen, Hui-Lu Zhang, Jie Liu, Jian-Lin Wu. Ultrasensitive quantification of trace amines based on N-phosphorylation labeling chip 2D LC-QQQ/MS[J]. Journal of Pharmaceutical Analysis, 2023, 13(3): 315-322. doi: 10.1016/j.jpha.2023.02.003

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Ultrasensitive quantification of trace amines based on N-phosphorylation labeling chip 2D LC-QQQ/MS

doi: 10.1016/j.jpha.2023.02.003

a. State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macao, 999078, China;
b. Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai, 200040, China

Funds:

This work was supported by the Science and Technology Development Fund, Macao, China (Grant No.: FDCT 0044/2018/AFJ).

Received Date: Apr. 18, 2022
Accepted Date: Feb. 09, 2023
Rev Recd Date: Jan. 29, 2023
Publish Date: Feb. 13, 2023

Abstract

Abstract

Trace amines (TAs) are metabolically related to catecholamine and associated with cancer and neurological disorders. Comprehensive measurement of TAs is essential for understanding pathological processes and providing proper drug intervention. However, the trace amounts and chemical instability of TAs challenge quantification. Here, diisopropyl phosphite coupled with chip two-dimensional (2D) liquid chromatography tandem triple-quadrupole mass spectrometry (LC-QQQ/MS) was developed to simultaneously determine TAs and associated metabolites. The results showed that the sensitivities of TAs increased up to 5520 times compared with those using nonderivatized LC-QQQ/MS. This sensitive method was utilized to investigate their alterations in hepatoma cells after treatment with sorafenib. The significantly altered TAs and associated metabolites suggested that phenylalanine and tyrosine metabolic pathways were related to sorafenib treatment in Hep3B cells. This sensitive method has great potential to elucidate the mechanism and diagnose diseases considering that an increasing number of physiological functions of TAs have been discovered in recent decades.
- Chip 2D LC-QQQ/MS,
- Trace amines,
- Derivatization,
- Hepatoma

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References(39)

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