Lipidome atlas of human myometrium reveals distinctive lipid signatures associated with adenomyosis: Combination of high-coverage lipidomics and mass spectrometry imaging

Shuo Liang; Jialin Liu; Maokun Liao; Dandan Liang; Yiyi Gong; Bo Zhang; Nan Zhao; Wei Song; Honghui Shi

doi:10.1016/j.jpha.2025.101197

Volume 15 Issue 9

Sep. 2025

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Shuo Liang, Jialin Liu, Maokun Liao, Dandan Liang, Yiyi Gong, Bo Zhang, Nan Zhao, Wei Song, Honghui Shi. Lipidome atlas of human myometrium reveals distinctive lipid signatures associated with adenomyosis: Combination of high-coverage lipidomics and mass spectrometry imaging[J]. Journal of Pharmaceutical Analysis, 2025, 15(9): 101197. doi: 10.1016/j.jpha.2025.101197

Citation:

Shuo Liang, Jialin Liu, Maokun Liao, Dandan Liang, Yiyi Gong, Bo Zhang, Nan Zhao, Wei Song, Honghui Shi. Lipidome atlas of human myometrium reveals distinctive lipid signatures associated with adenomyosis: Combination of high-coverage lipidomics and mass spectrometry imaging[J]. Journal of Pharmaceutical Analysis, 2025, 15(9): 101197. doi: 10.1016/j.jpha.2025.101197

Citation:

Shuo Liang, Jialin Liu, Maokun Liao, Dandan Liang, Yiyi Gong, Bo Zhang, Nan Zhao, Wei Song, Honghui Shi. Lipidome atlas of human myometrium reveals distinctive lipid signatures associated with adenomyosis: Combination of high-coverage lipidomics and mass spectrometry imaging[J]. Journal of Pharmaceutical Analysis, 2025, 15(9): 101197. doi: 10.1016/j.jpha.2025.101197

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Lipidome atlas of human myometrium reveals distinctive lipid signatures associated with adenomyosis: Combination of high-coverage lipidomics and mass spectrometry imaging

doi: 10.1016/j.jpha.2025.101197

a. Department of Obstetrics and Gynecology, National Clinical Research Center for Obstetric and Gynecologic Disease, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China;
b. Institute of Clinical Medicine, National Infrastructures for Translational Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China

Funds:

This study was supported by the National High Level Hospital Clinical Research Funding, China (Grant Nos.: 2022-PUMCH-A-204 and 2022-PUMCH-C-031) and the National Natural Science Foundation of China (Grant No.: 82274336).

Received Date: Oct. 12, 2024
Accepted Date: Jan. 12, 2025
Rev Recd Date: Jan. 02, 2025
Publish Date: Sep. 30, 2025

Abstract

Abstract

Adenomyosis is a common gynecological disease characterized by the invasion of endometrial glands and stroma into the myometrium of uterus, the pathological mechanism of which remains unclear yet. Disturbed lipid metabolism extensively affects abnormal cell proliferation and invasion in various diseases. However, the lipidome signature of human myometrium, which could be crucial in the development of adenomyosis, remains unknown. In this study, we generated the first lipidome profiling of human myometrium using a high-coverage and quantitative lipidomics approach based on ultra-performance liquid chromatography (UPLC) coupled with triple quadrupole (QqQ)-mass spectrometry (MS). A total of 317 lipid species were successfully quantified in the myometrial tissues from women with (n = 38) or without (n = 65) adenomyosis who underwent hysterectomy at Peking Union Medical College Hospital (Bejing, China). Up to 83 lipid species showed significant alternations in content between the two groups. These lipid aberrations involved multiple metabolic pathways, and emphasized inflammation, cell migration, and immune dysregulation upon adenomyosis. Moreover, receiver operating characteristic (ROC) curve analysis found that the combination of five lipid species could accurately distinguished the myometrial samples from women with and without adenomyosis with an area under the curve (AUC) of 0.906. Desorption electrospray ionization MS imaging (MSI) further underscored the heterogeneous distributions of these lipid markers in the adenomyosis lesion and adjacent myometrial tissue. Collectively, these results extremely improved our understanding on the molecular basis of adenomyosis, and could shed light on developing potential biomarkers and new therapeutic directions for adenomyosis.
- Adenomyosis,
- Myometrium,
- Lipidomics,
- Mass spectrometry imaging,
- Human uterus

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References(50)

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