Ningning Zhao, Shu Liu, Junpeng Xing, Zifeng Pi, Fengrui Song, Zhiqiang Liu. Trace determination and characterization of ginsenosides in rat plasma through magnetic dispersive solid-phase extraction based on core-shell polydopamine-coated magnetic nanoparticles[J]. Journal of Pharmaceutical Analysis, 2020, 10(1): 86-96.
Citation:
Ningning Zhao, Shu Liu, Junpeng Xing, Zifeng Pi, Fengrui Song, Zhiqiang Liu. Trace determination and characterization of ginsenosides in rat plasma through magnetic dispersive solid-phase extraction based on core-shell polydopamine-coated magnetic nanoparticles[J]. Journal of Pharmaceutical Analysis, 2020, 10(1): 86-96.
Ningning Zhao, Shu Liu, Junpeng Xing, Zifeng Pi, Fengrui Song, Zhiqiang Liu. Trace determination and characterization of ginsenosides in rat plasma through magnetic dispersive solid-phase extraction based on core-shell polydopamine-coated magnetic nanoparticles[J]. Journal of Pharmaceutical Analysis, 2020, 10(1): 86-96.
Citation:
Ningning Zhao, Shu Liu, Junpeng Xing, Zifeng Pi, Fengrui Song, Zhiqiang Liu. Trace determination and characterization of ginsenosides in rat plasma through magnetic dispersive solid-phase extraction based on core-shell polydopamine-coated magnetic nanoparticles[J]. Journal of Pharmaceutical Analysis, 2020, 10(1): 86-96.
Trace determination and characterization of ginsenosides in rat plasma through magnetic dispersive solid-phase extraction based on core-shell polydopamine-coated magnetic nanoparticles
National Center of Mass Spectrometry in Changchun and Jilin Province Key Laboratory of Chinese Medicine Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
University of Science and Technology of China, Hefei 230029, China
State Key Laboratory of Electroanalytiacl Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
Enrichment of trace bioactive constituents and metabolites from complex biological samples is chal-lenging. This study presented a one-pot synthesis of magnetic polydopamine nanoparticles (Fe3O4@-SiO2@PDA NPs) with multiple recognition sites for the magnetic dispersive solid-phase extraction (MDSPE) of ginsenosides from rat plasma treated with white ginseng. The extracted ginsenosides were characterized by combining an ultra-high-performance liquid chromatography coupled to a high-resolution mass spectrometry with supplemental UNIFI libraries. Response surface methodology was statistically used to optimize the extraction procedure of the ginsenosides. The reusability of Fe3O4@-SiO2@PDA NPs was also examined and the results showed that the recovery rate exceeded 80% after recycling 6 times. Furthermore, the proposed method showed greater enrichment efficiency and could rapidly determine and characterize 23 ginsenoside prototypes and metabolites from plasma. In com-parison, conventional methanol method can only detect 8 ginsenosides from the same plasma samples. The proposed approach can provide methodological reference for the trace determination and charac-terization of different bioactive ingredients and metabolites of traditional Chinese medicines and food.