Dongsheng Xu, Elena Sánchez-López, Qiqin Wang, Zhengjin Jiang, María Luisa Marina. Determination of L-norvaline and L-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column[J]. Journal of Pharmaceutical Analysis, 2020, 10(1): 70-77.
Citation:
Dongsheng Xu, Elena Sánchez-López, Qiqin Wang, Zhengjin Jiang, María Luisa Marina. Determination of L-norvaline and L-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column[J]. Journal of Pharmaceutical Analysis, 2020, 10(1): 70-77.
Dongsheng Xu, Elena Sánchez-López, Qiqin Wang, Zhengjin Jiang, María Luisa Marina. Determination of L-norvaline and L-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column[J]. Journal of Pharmaceutical Analysis, 2020, 10(1): 70-77.
Citation:
Dongsheng Xu, Elena Sánchez-López, Qiqin Wang, Zhengjin Jiang, María Luisa Marina. Determination of L-norvaline and L-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column[J]. Journal of Pharmaceutical Analysis, 2020, 10(1): 70-77.
Determination of L-norvaline and L-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column
Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871, Alcaláde Henares (Madrid), Spain
Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China
Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine &New Drug Research, Jinan University, Guangzhou, 510632, China
Instituto de Investigación Química"Andrés M. del Río"(IQAR), Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871, Alcaláde Henares (Madrid), Spain
An analytical methodology based on an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine (MQD)-silica hybrid monolithic column was developed for the enantioseparation of 9-fluorenylmethoxycarbonyl (FMOC) derivatized amino acids by nano-liquid chromatography. The mo-bile phase was optimized including the apparent pH, content of ACN, and concentration of the buffer to obtain a satisfactory enantioresolution performance. 27 FMOC derivatized amino acids including 19 protein and 8 non-protein amino acids were tested, and 19 out of them were enantiomerically discriminated obtaining baseline separation for 11 of them. Analytical characteristics of the method were evaluated for norvaline and tryptophan in terms of linearity, precision, accuracy, limits of detection (LOD) and quantitation (LOQ) showing good performance to be applied to the enantiomeric determination of these amino acids in dietary supplements. LOD and LOQ values were 9.3 and 31μM for norvaline en-antiomers and 7.5 and 25μM for tryptophan enantiomers, respectively. The contents of D-norvaline and D-tryptophan were below their respective LODs in all the analyzed samples. Quantitation of L-tryptophan and L-norvaline showed good agreement with the labeled contents except for one sample which did not show presence of L-norvaline, contrary to the label indication.
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Dongsheng Xu, Elena Sánchez-López, Qiqin Wang, Zhengjin Jiang, María Luisa Marina. Determination of L-norvaline and L-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column[J]. Journal of Pharmaceutical Analysis, 2020, 10(1): 70-77.
Dongsheng Xu, Elena Sánchez-López, Qiqin Wang, Zhengjin Jiang, María Luisa Marina. Determination of L-norvaline and L-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column[J]. Journal of Pharmaceutical Analysis, 2020, 10(1): 70-77.
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