Cristina Panella, Rosella Ferretti, Adriano Casulli, Roberto Cirilli. Temperature and eluent composition effects on enantiomer separation of carvedilol by high-performance liquid chromatography on immobilized amylose-based chiral stationary phases[J]. Journal of Pharmaceutical Analysis, 2019, 9(5): 324-331.
Citation:
Cristina Panella, Rosella Ferretti, Adriano Casulli, Roberto Cirilli. Temperature and eluent composition effects on enantiomer separation of carvedilol by high-performance liquid chromatography on immobilized amylose-based chiral stationary phases[J]. Journal of Pharmaceutical Analysis, 2019, 9(5): 324-331.
Cristina Panella, Rosella Ferretti, Adriano Casulli, Roberto Cirilli. Temperature and eluent composition effects on enantiomer separation of carvedilol by high-performance liquid chromatography on immobilized amylose-based chiral stationary phases[J]. Journal of Pharmaceutical Analysis, 2019, 9(5): 324-331.
Citation:
Cristina Panella, Rosella Ferretti, Adriano Casulli, Roberto Cirilli. Temperature and eluent composition effects on enantiomer separation of carvedilol by high-performance liquid chromatography on immobilized amylose-based chiral stationary phases[J]. Journal of Pharmaceutical Analysis, 2019, 9(5): 324-331.
Temperature and eluent composition effects on enantiomer separation of carvedilol by high-performance liquid chromatography on immobilized amylose-based chiral stationary phases
Centro Nazionale per il Controllo e la Valutazione dei Farmaci, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
European Union Reference Laboratory for the Parasites, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
World Health Organization Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis (in Animals and Humans), Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
Carvedilol is a chiral drug with potent antihypertensive and antianginal activities. Although it is clinically used as a racemic mixture, its enantiomers show different pharmacokinetic and pharmacodynamic profiles. Here, the direct chiral separation of racemic drug by high performance liquid chromatography using two immobilized-type amylose-based chiral stationary phases is presented. Some chromato-graphic parameters, such as retention and selectivity, were determined under multimodal eluent con-ditions and different temperatures. A temperature-dependent inversion of the elution order of enantiomers was observed in the operative temperature range of chiral chromatographic support. Finally, an effective direct enantioselective method was successfully applied to the separation of the enantiomers of carvedilol on a semipreparative scale.