Yorick Janssens, Frederick Verbeke, Nathan Debunne, Evelien Wynendaele, Kathelijne Peremans, Bart De Spiegeleer. Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry[J]. Journal of Pharmaceutical Analysis, 2018, 8(1): 69-74.
Citation:
Yorick Janssens, Frederick Verbeke, Nathan Debunne, Evelien Wynendaele, Kathelijne Peremans, Bart De Spiegeleer. Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry[J]. Journal of Pharmaceutical Analysis, 2018, 8(1): 69-74.
Yorick Janssens, Frederick Verbeke, Nathan Debunne, Evelien Wynendaele, Kathelijne Peremans, Bart De Spiegeleer. Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry[J]. Journal of Pharmaceutical Analysis, 2018, 8(1): 69-74.
Citation:
Yorick Janssens, Frederick Verbeke, Nathan Debunne, Evelien Wynendaele, Kathelijne Peremans, Bart De Spiegeleer. Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry[J]. Journal of Pharmaceutical Analysis, 2018, 8(1): 69-74.
Drug Quality and Registration (DruQuaR) Group,Faculty of Pharmaceutical Sciences,Ghent University,Ottergemsesteenweg 460,B-9000 Ghent,Belgium
Department of Veterinary Medical Imaging and Smal Animal Orthopaedics,Faculty of Veterinary Medicine,Ghent University,Salisburylaan 133,B-9820 Merelbeke,Belgium
Funds:
The authors thank the 'Research Foundation–Flanders
the 'Institute for the Promotion of Innovation through Science and Technology in Flanders
Five different quorum sensing peptides (QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1% (m/v) formic acid as mobile phase. Electrospray ionization (ESI) ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quan-tification was performed using total ion current (TIC) spectra. Non-iodinated peptides and mono-and di-iodinated peptides (NIP, MIP and DIP respectively) were well separated and eluted in that order. De-pending on the used iodination method, iodination yields varied from low (2%) to high (57%).
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