Volume 6 Issue 6
Dec.  2016
Turn off MathJax
Article Contents
Pradeepa Krishnappa, Krishna Venkatarangaiah, Venkatesh, Santosh Kumar Shimoga Rajanna, Rebijith Kayattukandy Balan. Wound healing activity of Delonix elata stem bark extract and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside in rats☆[J]. Journal of Pharmaceutical Analysis, 2016, 6(6): 389-395.
Citation: Pradeepa Krishnappa, Krishna Venkatarangaiah, Venkatesh, Santosh Kumar Shimoga Rajanna, Rebijith Kayattukandy Balan. Wound healing activity of Delonix elata stem bark extract and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside in rats☆[J]. Journal of Pharmaceutical Analysis, 2016, 6(6): 389-395.

Wound healing activity of Delonix elata stem bark extract and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside in rats☆

Funds:

This work is partially supported by the Department of Biotechnology, New Delhi, India

  • Publish Date: Dec. 10, 2016
  • Delonix elata L. is a Ceasalpinaceae species and is traditionally used in India for treatment of skin diseases, liver diseases and rheumatic problems. However, systematic evaluation of its wound healing activity is lacking. Thus, in the present study, we aimed to assess the wound healing activity of D. elata stem bark extract (DSE) and its isolated constituent quercetin-3-rhamnopyranosyl-(1-6) glucopyranoside (QRPG) in rats. The formulations effects on wound healing were assessed by the wound contraction rate, epithelialization period, tensile strength, content of the hydroxyproline, hexosamine and uronic acid in granulation tissue, histopathological studies and Col 1α(I) expression level in wound tissue by reverse transcription polymerase chain reaction (RT-PCR) study. The topical application of DSE ointment caused faster epithelialization, significant wound contraction (100%), and better tensile strength (710.5 ± 10.5 g/cm2), while QRPG showed wound epithelialization with 98.2%contraction, better than that of the control group (78.18%). The biochemical analysis of granulation tissue revealed that DSE and QRPG significantly increased hydroxyproline, hexosamine and uronic acid content. A significant increase in the expression of Col 1α(I) was observed in the wound tissue of DSE and QRPG treated rats. DSE and QRPG were shown to enhance wound healing by increasing collagen synthesis through up-regulation of Col 1α(I), thus validating ethnomedicinal uses.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (84) PDF downloads(1) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return