Volume 6 Issue 4
Aug.  2016
Turn off MathJax
Article Contents
Atanu Singha Roy n, Amit Kumar Dinda, Nitin Kumar Pandey, Swagata Dasgupta. Effects of urea, metal ions and surfactants on the binding of baicalein with bovine serum albumin$[J]. Journal of Pharmaceutical Analysis, 2016, 6(4): 256-267.
Citation: Atanu Singha Roy n, Amit Kumar Dinda, Nitin Kumar Pandey, Swagata Dasgupta. Effects of urea, metal ions and surfactants on the binding of baicalein with bovine serum albumin$[J]. Journal of Pharmaceutical Analysis, 2016, 6(4): 256-267.

Effects of urea, metal ions and surfactants on the binding of baicalein with bovine serum albumin$

Funds:

Swagata Dasgupta is grateful to Department of Science and Technology (DST

Government of India for financial support

  • Publish Date: Aug. 10, 2016
  • The interaction of baicalein with bovine serum albumin (BSA) was investigated with the help of spec-troscopic and molecular docking studies. The binding affinity of baicalein towards BSA was estimated to be in order of 105 M?1 from fluorescence quenching studies. NegativeΔH° (?5.6670.14 kJ/mol) and positive (ΔS°) ( t 79.96 7 0.65 J/mol K) indicate the presence of electrostatic interactions along with the hydrophobic forces that result in a positiveΔS°. The hydrophobic association of baicalein with BSA di-minishes in the presence of sodium dodecyl sulfate (SDS) due to probable hydrophobic association of baicalein with SDS, resulting in a negativeΔS° ( ? 40.65 7 0.87 J/mol K). Matrix-assisted laser desorption ionization/time of flight (MALDI–TOF) experiments indicate a 1:1 complexation between baicalein and BSA. The unfolding and refolding phenomena of BSA were investigated in the absence and presence of baicalein using steady-state and fluorescence lifetime measurements. It was observed that the presence of urea ruptured the non-covalent interaction between baicalein and BSA. The presence of metal ions (Ag t , Mg2 t , Ni2 t , Mn2 t , Co2 t and Zn2 t ) increased the binding affinity of ligand towards BSA. The changes in conformational aspects of BSA after ligand binding were also investigated using circular di-chroism (CD) and Fourier transform infrared (FT-IR) spectroscopic techniques. Site selectivity studies following molecular docking analyses indicated the binding of baicalein to site 1 (subdomain IIA) of BSA.&2016 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. This is an open access article.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (98) PDF downloads(1) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return