Rongshan Li, Ruixue Ran, Quansheng Li, Yurong Huang, Yuan Gu, Duanyun Si. Development of an LC-MS/MS method for the quantitation of deoxyglycychloxazol in rat plasma and its application in pharmacokinetic study$[J]. Journal of Pharmaceutical Analysis, 2016, 6(3): 184-189.
Citation:
Rongshan Li, Ruixue Ran, Quansheng Li, Yurong Huang, Yuan Gu, Duanyun Si. Development of an LC-MS/MS method for the quantitation of deoxyglycychloxazol in rat plasma and its application in pharmacokinetic study$[J]. Journal of Pharmaceutical Analysis, 2016, 6(3): 184-189.
Rongshan Li, Ruixue Ran, Quansheng Li, Yurong Huang, Yuan Gu, Duanyun Si. Development of an LC-MS/MS method for the quantitation of deoxyglycychloxazol in rat plasma and its application in pharmacokinetic study$[J]. Journal of Pharmaceutical Analysis, 2016, 6(3): 184-189.
Citation:
Rongshan Li, Ruixue Ran, Quansheng Li, Yurong Huang, Yuan Gu, Duanyun Si. Development of an LC-MS/MS method for the quantitation of deoxyglycychloxazol in rat plasma and its application in pharmacokinetic study$[J]. Journal of Pharmaceutical Analysis, 2016, 6(3): 184-189.
Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics Theranostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, China
State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China
Deoxyglycychloxazol (TY501) is a glycyrrhetinic acid derivative which exhibits high anti-inflammatory activity and reduced pseudoaldosteronism compared to glycyrrhetinic acid. In this study, a sensitive and rapid liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was established for the quantitation of TY501 in rat plasma. Plasma samples were treated by precipitating protein with methanol and supernatants were separated by a Symmetry C8 column with the mobile phase consisting of me-thanol and 10 mM ammonium formate (containing 0.1%of formic acid) (90:10, v/v). The selected reaction monitoring (SRM) transitions were performed at m/z 647.4-191.2 for TY501 and m/z 473.3-143.3 for astragaloside aglycone (IS) in the positive ion mode with atmospheric pressure chemical ionization (APCI) source. Calibration curve was linear over the concentration range of 5–5000 ng/mL. The lower limit of quantification was 5 ng/mL. The mean recovery was over 88%. The intra-and inter-day precisions were lower than 6.0% and 12.8%, respectively, and the accuracy was within 71.3%. TY501 was stable under usual storage conditions and handling procedure. The validated method has been successfully applied to a pharmacokinetic study after oral administration of TY501 to rats at a dosage of 10 mg/kg.