Shi-Ying Dai, Shi-Ting Qiu, Wei Wu, Chun-Mei Fu. Development and validation of an RP-HPLC method for simultaneous determination of Ramipril and Amlodipine in tablets[J]. Journal of Pharmaceutical Analysis, 2013, (6): 440-446.
Citation:
Shi-Ying Dai, Shi-Ting Qiu, Wei Wu, Chun-Mei Fu. Development and validation of an RP-HPLC method for simultaneous determination of Ramipril and Amlodipine in tablets[J]. Journal of Pharmaceutical Analysis, 2013, (6): 440-446.
Shi-Ying Dai, Shi-Ting Qiu, Wei Wu, Chun-Mei Fu. Development and validation of an RP-HPLC method for simultaneous determination of Ramipril and Amlodipine in tablets[J]. Journal of Pharmaceutical Analysis, 2013, (6): 440-446.
Citation:
Shi-Ying Dai, Shi-Ting Qiu, Wei Wu, Chun-Mei Fu. Development and validation of an RP-HPLC method for simultaneous determination of Ramipril and Amlodipine in tablets[J]. Journal of Pharmaceutical Analysis, 2013, (6): 440-446.
Key Laboratory of Drug Targeting and Drug Delivery System, Ministry of Education, West China School of Pharmacy, Sichuan University, No. 17, Block 3, Southern Renmin Road, Chengdu 610041, China
An RP-HPLC method for the simultaneous determination of and Amlodipine (AL)Ramipril (RP) in tablets was developed and validated by Chinese Pharmacopoeia 2010. The linearity of the proposed method was investigated in the range of 0.01-0.25 mg/mL (r2 ? 0.9998) for RP and 0.014-0.36 mg/mL (r2 ? 0.9997) for AL. The limits of detection (LOD) were 0.06μg/mL and 0.02μg/mL for RP and AL, and the limits of quantitation (LOQ) were 0.2μg/mL and 0.07μg/mL, respectively. Some major impurities and degradation products did not disturb the detection of RP and AL and the assay can thus be considered stability-indicating.
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