Jing-Jing Liu, Juan Zhang, Zi-Lin Chen. Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-b-CD as a chiral selector[J]. Journal of Pharmaceutical Analysis, 2013, (5): 349-353.
Citation:
Jing-Jing Liu, Juan Zhang, Zi-Lin Chen. Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-b-CD as a chiral selector[J]. Journal of Pharmaceutical Analysis, 2013, (5): 349-353.
Jing-Jing Liu, Juan Zhang, Zi-Lin Chen. Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-b-CD as a chiral selector[J]. Journal of Pharmaceutical Analysis, 2013, (5): 349-353.
Citation:
Jing-Jing Liu, Juan Zhang, Zi-Lin Chen. Chiral separation of bavachinin in Fructus Psoraleae and rat plasma by liquid chromatography using permethylated-b-CD as a chiral selector[J]. Journal of Pharmaceutical Analysis, 2013, (5): 349-353.
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China
A simple, sensitive and selective method of high-performance liquid chromatography (HPLC) has been successfully developed for separation of bavachinin enantiomers in Fructus Psoraleae and rat plasma. The separation and detection conditions of HPLC were optimized. Chiral bavachinin were separated with the mobile phase of methanol and water (70:30, v/v) at a flow rate of 1.0 mL/min. The linear ranges were in the range of 20-1000 mg/mL. The detection limits were tested as 4 ng/mL and 6 ng/mL for (t)-bavachinin and (à)-bavachinin, respectively. The method has been applied to analyze chiral bavachinin in rat plasma. HPLC-MS method was used to test the accuracy.
DownLoad: