A novel strategy based on the dielectric barrier discharge plasma for rapid eliminating the carryover associated with μPESI-MS/MS system

Qian Liu; Simin Zhang; Xiangyang Qu; Yunhui Xing; Zhenwei Xiao; Shicheng Fan; Janshon Zhu; Min Huang; Huichang Bi

doi:10.1016/j.jpha.2024.101017

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Qian Liu, Simin Zhang, Xiangyang Qu, Yunhui Xing, Zhenwei Xiao, Shicheng Fan, Janshon Zhu, Min Huang, Huichang Bi. A novel strategy based on the dielectric barrier discharge plasma for rapid eliminating the carryover associated with μPESI-MS/MS system[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101017

Citation:

Qian Liu, Simin Zhang, Xiangyang Qu, Yunhui Xing, Zhenwei Xiao, Shicheng Fan, Janshon Zhu, Min Huang, Huichang Bi. A novel strategy based on the dielectric barrier discharge plasma for rapid eliminating the carryover associated with μPESI-MS/MS system[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101017

Citation:

Qian Liu, Simin Zhang, Xiangyang Qu, Yunhui Xing, Zhenwei Xiao, Shicheng Fan, Janshon Zhu, Min Huang, Huichang Bi. A novel strategy based on the dielectric barrier discharge plasma for rapid eliminating the carryover associated with μPESI-MS/MS system[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.101017

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A novel strategy based on the dielectric barrier discharge plasma for rapid eliminating the carryover associated with μPESI-MS/MS system

doi: 10.1016/j.jpha.2024.101017

Qian Liu^a,b,c,
Simin Zhang^b,
Xiangyang Qu^b,
Yunhui Xing^b,
Zhenwei Xiao^b,
Shicheng Fan^a,
Janshon Zhu^{c
,
,},
Min Huang^{b
,
,},
Huichang Bi^{a,b,d
,
,}

a. Guangdong Provincial Key Laboratory of New Drug Screening & NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Guangdong, China, 510515;
b. Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China, 510080;
c. Guangdong RangerBio Technologies Co., Ltd., Dongguan, Guangdong, China, 523000;
d. The State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University, Shenzhen, China 518055

Funds:

The authors would like to acknowledge the financial supports provided by the National Natural Science Foundation of China [grant number: 82025034 and 81973392], the Science and Technology Project of Guizhou province (project number: ZK [2022] General 410), the Social Development Technology Project of Dongguan (project number: 20211800907893) and the Shenzhen Science and Technology Program [No. KQTD20190929174023858].

Received Date: Jan. 12, 2024
Accepted Date: May 30, 2024
Rev Recd Date: May 22, 2024
Available Online: Jun. 13, 2024

Abstract

Abstract

In mass spectrometry (MS) analysis, carryover is a common and unavoidable problem. It causes over-estimation of analyte levels, which significantly influences accuracy and precision. Therefore, there are strict limits on carryover in MS-based assays. The conventional capillary-based electrospray ionization (ESI) restricts the use of MS for real-time monitoring, where a prompt response is crucial. Recently, we have developed a micro probe electrospray ionization (μPESI) coupled with MS (μPESI-MS) technology, enabling MS analysis within 40 seconds without a chromatographic column [1]. However, the challenge of carryover arises when trying to make the micro probe reusable for resource-saving purposes. Considering that dielectric barrier discharge (DBD) plasma is effective in degrading contaminants in water and gas [2], we hypothesized the eventual use of the DBD plasma to “wash” the micro probe. Then, a DBD plasma wash tube (DBD-WT) was introduced for rapid carryover elimination in μPESI-MS analysis.

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