Optical biosensing of monkeypox virus using novel recombinant silica-binding proteins for site-directed antibody immobilization

Xixi Song; Ying Tao; Sumin Bian; Mohamad Sawan

doi:10.1016/j.jpha.2024.100995

Article Contents

Article Navigation > Journal of Pharmaceutical Analysis > 2024 > Accepted Manu

Xixi Song, Ying Tao, Sumin Bian, Mohamad Sawan. Optical biosensing of monkeypox virus using novel recombinant silica-binding proteins for site-directed antibody immobilization[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.100995

Citation:

Xixi Song, Ying Tao, Sumin Bian, Mohamad Sawan. Optical biosensing of monkeypox virus using novel recombinant silica-binding proteins for site-directed antibody immobilization[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.100995

Citation:

Xixi Song, Ying Tao, Sumin Bian, Mohamad Sawan. Optical biosensing of monkeypox virus using novel recombinant silica-binding proteins for site-directed antibody immobilization[J]. Journal of Pharmaceutical Analysis. doi: 10.1016/j.jpha.2024.100995

PDF( 7173 KB)

Optical biosensing of monkeypox virus using novel recombinant silica-binding proteins for site-directed antibody immobilization

doi: 10.1016/j.jpha.2024.100995

CenBRAIN Neurotech, School of Engineering, Westlake University, Hangzhou, 310030, China

Funds:

This research was supported by Westlake University, China (Startup funds), the Research Center for Industries of the Future of Westlake University, China (Grant No.: WU2022C040), and the National Natural Science Foundation of China (Grant No.: 82104122).

Received Date: Jan. 30, 2024
Accepted Date: Apr. 29, 2024
Rev Recd Date: Apr. 26, 2024
Available Online: May 10, 2024

Abstract

Abstract

The efficient immobilization of capture antibodies is crucial for timely pathogen detection during global pandemic outbreaks. Therefore, we proposed a silica-binding protein featuring core functional domains (cSP). It comprises a peptide with a silica-binding tag designed to adhere to silica surfaces and tandem protein G fragments for effective antibody capture. This innovation facilitates precise site-directed immobilization of antibodies onto silica surfaces. We applied cSP to silica-coated optical fibers, creating a fiber-optic biolayer interferometer (FO-BLI) biosensor capable of monitoring the Monkeypox Virus (MPXV) Protein A29L in spiked clinical samples to rapidly detect the MPXV. The cSP-based FO-BLI biosensor for MPXV demonstrated a limit of detection (LOD) of 0.62 ng/mL in buffer, comparable to the 0.52 ng/mL LOD achieved using a conventional streptavidin-based FO-BLI biosensor. Furthermore, it achieved LODs of 0.77 ng/mL in spiked serum and 0.80 ng/mL in spiked saliva, exhibiting no cross-reactivity with other viral antigens. The MPXV detection process was completed within 14 min. We further proposed a cSP-based multi-virus biosensor strategy capable of detecting various pandemic strains, such as MPXV, the latest coronavirus disease (COVID) variants, and influenza A protein, to extend its versatility. The proposed cSP-modified FO-BLI biosensor has a high potential for rapidly and accurately detecting MPXV antigens, making valuable contributions to epidemiological studies.
- Site-directed immobilization,
- Silica-binding proteins,
- Optical biosensing,
- Monkeypox virus,
- Spiked clinical samples,
- Multi-virus biosensor

FullText(HTML)

References(33)

References

[1]	E. Morales-Narváez, C. Dincer, The impact of biosensing in a pandemic outbreak: COVID-19, Biosens. Bioelectron. 163 (2020), 112274.
[2]	W.H. Organization, 2022–23 Mpox (Monkeypox) outbreak: Global trends. https://worldhealthorg.shinyapps.io/mpx_global/. (Accessed 19 December 2023).
[3]	F.-M. Lum, A. Torres-Ruesta, M.Z. Tay, et al., Monkeypox: Disease epidemiology, host immunity and clinical interventions, Nat. Rev. Immunol. 22 (2022) 597-613.
[4]	I. Gul, C. Liu, X. Yuan, et al., Current and perspective sensing methods for monkeypox virus, Bioengineering (Basel) 9 (2022), 571.
[5]	P. Halvaei, S. Zandi, M. Zandi, Biosensor as a novel alternative approach for early diagnosis of monkeypox virus, Int. J. Surg. 109 (2023) 50-52.
[6]	G. Rabbani, M.E. Khan, A.U. Khan, et al., Label-free and ultrasensitive electrochemical transferrin detection biosensor based on a glassy carbon electrode and gold nanoparticles, Int. J. Biol. Macromol. 256 (2024), 128312.
[7]	G. Rabbani, M.E. Khan, E. Ahmad, et al., Serum CRP biomarker detection by using carbon nanotube field-effect transistor (CNT-FET) immunosensor, Bioelectrochemistry 153 (2023), 108493.
[8]	Y. Zheng, S. Bian, J. Sun, et al., Label-free LSPR-vertical microcavity biosensor for on-site SARS-CoV-2 detection, Biosensors 12 (2022), 151.
[9]	M. Mathelie-Guinlet, T. Cohen-Bouhacina, I. Gammoudi, et al., Silica nanoparticles-assisted electrochemical biosensor for the rapid, sensitive and specific detection of Escherichia coli, Sens. Actuat. B Chem. 292 (2019) 314-320.
[10]	X. Zhang, Y. Zhang, X. Zhang, et al., Interface design and dielectric response behavior of SiO₂/PB composites with low dielectric constant and ultra-low dielectric loss, Surf. Interfaces 22 (2021), 100807.
[11]	L.S. Puumala, S.M. Grist, J.M. Morales, et al., Biofunctionalization of multiplexed silicon photonic biosensors, Biosensors (Basel) 13 (2023), 53.
[12]	A. Sadiki, S.R. Vaidya, M. Abdollahi, et al., Site-specific conjugation of native antibody, Antib. Ther. 3 (2020) 271-284.
[13]	D. Jeon, J.-C. Pyun, J. Jose, et al., A regenerative immunoaffinity layer based on the outer membrane of Z-domains autodisplaying E. coli for immunoassays and immunosensors, Sensors (Basel) 18 (2018), 4030.
[14]	H.G. Lee, S. Kang, J.S. Lee, Binding characteristics of staphylococcal protein A and streptococcal protein G for fragment crystallizable portion of human immunoglobulin G, Comput. Struct. Biotechnol. J. 19 (2021) 3372-3383.
[15]	X. Song, Z. Fredj, Y. Zheng, et al., Biosensors for waterborne virus detection: Challenges and strategies, J. Pharm. Anal. 13 (2023) 1252-1268.
[16]	S. Kim, K.I. Joo, B.H. Jo, et al., Stability-controllable self-immobilization of carbonic anhydrase fused with a silica-binding tag onto diatom biosilica for enzymatic CO₂ capture and utilization, ACS Appl. Mater. Interfaces 12 (2020) 27055-27063.
[17]	S. Bian, M. Shang, M. Sawan, Rapid biosensing SARS-CoV-2 antibodies in vaccinated healthy donors, Biosens. Bioelectron. 204 (2022), 114054.
[18]	S. Bian, M. Shang, Y. Tao, et al., Dynamic profiling and prediction of antibody response to SARS-CoV-2 booster-inactivated vaccines by microsample-driven biosensor and machine learning, Vaccines 12 (2024), 352.
[19]	Y. Tao, S. Bian, P. Wang, et al., Rapid optical biosensing of SARS-CoV-2 spike proteins in artificial samples, Sensors (Basel) 22 (2022), 3768.
[20]	S. Bian, Y. Tao, Z. Zhu, et al., On-site biolayer interferometry-based biosensing of carbamazepine in whole blood of epileptic patients, Biosensors (Basel) 11 (2021), 516.
[21]	B. Webb, A. Sali, Comparative protein structure modeling using MODELLER, Curr. Protoc. Bioinform. 54 (2016) 5.6.1-5.6.37.
[22]	A. Waterhouse, M. Bertoni, S. Bienert, et al., SWISS-MODEL: Homology modelling of protein structures and complexes, Nucleic Acids Res. 46 (2018) W296-W303.
[23]	G. Studer, C. Rempfer, A.M. Waterhouse, et al., QMEANDisCo-distance constraints applied on model quality estimation, Bioinformatics 36 (2020) 1765-1771.
[24]	Schrodinger, LLC, The PyMOL Molecular Graphics System, Version 2.0.
[25]	C. Liu, D.L. Steer, H. Song, et al., Superior binding of proteins on a silica surface: Physical insight into the synergetic contribution of polyhistidine and a silica-binding peptide, J. Phys. Chem. Lett. 13 (2022) 1609-1616.
[26]	C. Zhang, L. Liu, H. Li, et al., An oriented antibody immobilization based electrochemical platform for detection of leptin in human with different body mass index, Sens. Actuat. B Chem. 353 (2022), 131074.
[27]	J.B. Fishman, E.A. Berg, Protein A and protein G purification of antibodies, Cold Spring Harb Protoc. (2019).
[28]	Z. Zhang, H. Jiang, S. Jiang, et al., Rapid detection of the monkeypox virus genome and antigen proteins based on surface-enhanced Raman spectroscopy, ACS Appl. Mater. Interfaces 15 (2023) 34419-34426.
[29]	L. Ye, X. Lei, X. Xu, et al., Gold-based paper for antigen detection of monkeypox virus, Analyst 148 (2023) 985-994.
[30]	P. Cao, X. Lai, R. Zhang, et al., Fluorescent immunochromatographic assay (FICA) for monkeypox virus, Anal. Lett. (2023) 1-14.
[31]	C. Wang, Q. Yu, J. Li, et al., Colorimetric-fluorescent dual-signal enhancement immunochromatographic assay based on molybdenum disulfide-supported quantum dot nanosheets for the point-of-care testing of monkeypox virus, Chem. Eng. J. 472 (2023), 144889.
[32]	Y. Zheng, X. Song, Z. Fredj, et al., Challenges and perspectives of multi-virus biosensing techniques: A review, Anal. Chim. Acta 1244 (2023), 340860.
[33]	T. Ikeda, A. Kuroda, Why does the silica-binding protein “Si-tag” bind strongly to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid surfaces, Colloids Surf. B Biointerfaces 86 (2011) 359-363.