Modern approaches for detection of volatile organic compounds in metabolic studies focusing on pathogenic bacteria: Current state of the art

Karolina &#379;uchowska; Wojciech Filipiak

doi:10.1016/j.jpha.2023.11.005

Volume 14 Issue 4

Apr. 2024

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Karolina Żuchowska, Wojciech Filipiak. Modern approaches for detection of volatile organic compounds in metabolic studies focusing on pathogenic bacteria: Current state of the art[J]. Journal of Pharmaceutical Analysis, 2024, 14(4): 100898. doi: 10.1016/j.jpha.2023.11.005

Citation:

Karolina Żuchowska, Wojciech Filipiak. Modern approaches for detection of volatile organic compounds in metabolic studies focusing on pathogenic bacteria: Current state of the art[J]. Journal of Pharmaceutical Analysis, 2024, 14(4): 100898. doi: 10.1016/j.jpha.2023.11.005

Citation:

Karolina Żuchowska, Wojciech Filipiak. Modern approaches for detection of volatile organic compounds in metabolic studies focusing on pathogenic bacteria: Current state of the art[J]. Journal of Pharmaceutical Analysis, 2024, 14(4): 100898. doi: 10.1016/j.jpha.2023.11.005

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Modern approaches for detection of volatile organic compounds in metabolic studies focusing on pathogenic bacteria: Current state of the art

doi: 10.1016/j.jpha.2023.11.005

Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, Poland

Funds:

This research was funded by the National Science Centre, Poland (Project No.: 2017/26/D/NZ6/00136).

Received Date: Jun. 27, 2023
Accepted Date: Nov. 15, 2023
Rev Recd Date: Oct. 03, 2023
Publish Date: Nov. 28, 2023

Abstract

Abstract

Pathogenic microorganisms produce numerous metabolites, including volatile organic compounds (VOCs). Monitoring these metabolites in biological matrices (e.g., urine, blood, or breath) can reveal the presence of specific microorganisms, enabling the early diagnosis of infections and the timely implementation of targeted therapy. However, complex matrices only contain trace levels of VOCs, and their constituent components can hinder determination of these compounds. Therefore, modern analytical techniques enabling the non-invasive identification and precise quantification of microbial VOCs are needed. In this paper, we discuss bacterial VOC analysis under in vitro conditions, in animal models and disease diagnosis in humans, including techniques for offline and online analysis in clinical settings. We also consider the advantages and limitations of novel microextraction techniques used to prepare biological samples for VOC analysis, in addition to reviewing current clinical studies on bacterial volatilomes that address inter-species interactions, the kinetics of VOC metabolism, and species- and drug-resistance specificity.
- Volatile organic compounds,
- Pathogenic bacteria metabolites,
- Metabolomics,
- Microextraction techniques,
- Gas chromatography-mass spectrometry,
- in vivo breath analysis,
- In vitro model

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References(140)

References

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