On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay

Junxia Gao; Tianyi Zhang; Yihua Fang; Ying Zhao; Mei Yang; Li Zhao; Ye Li; Jun Huang; Guonian Zhu; Yirong Guo

doi:10.1016/j.jpha.2023.09.011

Volume 14 Issue 2

Feb. 2024

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Article Navigation > Journal of Pharmaceutical Analysis > 2024 > 14(2): 276-283

Junxia Gao, Tianyi Zhang, Yihua Fang, Ying Zhao, Mei Yang, Li Zhao, Ye Li, Jun Huang, Guonian Zhu, Yirong Guo. On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay[J]. Journal of Pharmaceutical Analysis, 2024, 14(2): 276-283. doi: 10.1016/j.jpha.2023.09.011

Citation:

Junxia Gao, Tianyi Zhang, Yihua Fang, Ying Zhao, Mei Yang, Li Zhao, Ye Li, Jun Huang, Guonian Zhu, Yirong Guo. On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay[J]. Journal of Pharmaceutical Analysis, 2024, 14(2): 276-283. doi: 10.1016/j.jpha.2023.09.011

Citation:

Junxia Gao, Tianyi Zhang, Yihua Fang, Ying Zhao, Mei Yang, Li Zhao, Ye Li, Jun Huang, Guonian Zhu, Yirong Guo. On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay[J]. Journal of Pharmaceutical Analysis, 2024, 14(2): 276-283. doi: 10.1016/j.jpha.2023.09.011

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On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay

doi: 10.1016/j.jpha.2023.09.011

a. Institute of Pesticide and Environmental Toxicology, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, 310058, China;
b. Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, 310008, China;
c. Shanghai Agricultural Technology Extension Service Center, Shanghai, 201103, China;
d. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 311200, China

Funds:

This work was supported by grants from Shanghai Agriculture Applied Technology Development Program, China (Grant No.: 2020-02-08-00-08-F01456) and the Key Research and Development Program of Zhejiang Province, China (Grant No.: 2020C02024-2).

Received Date: Apr. 27, 2023
Accepted Date: Sep. 18, 2023
Rev Recd Date: Sep. 04, 2023
Publish Date: Feb. 29, 2024

Abstract

Abstract

The application of pesticides (mostly insecticides and fungicides) during the tea-planting process will undoubtedly increase the dietary risk associated with drinking tea. Thus, it is necessary to ascertain whether pesticide residues in tea products exceed the maximum residue limits. However, the complex matrices present in tea samples comprise a major challenge in the analytical detection of pesticide residues. In this study, nine types of lateral flow immunochromatographic strips (LFICSs) were developed to detect the pesticides of interest (fenpropathrin, chlorpyrifos, imidacloprid, thiamethoxam, acetamiprid, carbendazim, chlorothalonil, pyraclostrobin, and iprodione). To reduce the interference of tea substrates on the assay sensitivity, the pretreatment conditions for tea samples, including the extraction solvent, extraction time, and purification agent, were optimized for the simultaneous detection of these pesticides. The entire testing procedure (including pretreatment and detection) could be completed within 30 min. The detected results of authentic tea samples were confirmed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), which suggest that the LFICS coupled with sample rapid pretreatment can be used for on-site rapid screening of the target pesticide in tea products prior to their market release.
- Lateral flow immunoassay,
- Rapid detection,
- Pesticide multi-residue,
- Tea matrix,
- Sample rapid pretreatment

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References(26)

References

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