Chemical derivatization strategies for enhancing the HPLC analytical performance of natural active triterpenoids

Xiao-Feng Huang; Ying Xue; Li Yong; Tian-Tian Wang; Pei Luo; Lin-Sen Qing

doi:10.1016/j.jpha.2023.07.004

Volume 14 Issue 3

Mar. 2024

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Article Navigation > Journal of Pharmaceutical Analysis > 2024 > 14(3): 295-307

Xiao-Feng Huang, Ying Xue, Li Yong, Tian-Tian Wang, Pei Luo, Lin-Sen Qing. Chemical derivatization strategies for enhancing the HPLC analytical performance of natural active triterpenoids[J]. Journal of Pharmaceutical Analysis, 2024, 14(3): 295-307. doi: 10.1016/j.jpha.2023.07.004

Citation:

Xiao-Feng Huang, Ying Xue, Li Yong, Tian-Tian Wang, Pei Luo, Lin-Sen Qing. Chemical derivatization strategies for enhancing the HPLC analytical performance of natural active triterpenoids[J]. Journal of Pharmaceutical Analysis, 2024, 14(3): 295-307. doi: 10.1016/j.jpha.2023.07.004

Citation:

Xiao-Feng Huang, Ying Xue, Li Yong, Tian-Tian Wang, Pei Luo, Lin-Sen Qing. Chemical derivatization strategies for enhancing the HPLC analytical performance of natural active triterpenoids[J]. Journal of Pharmaceutical Analysis, 2024, 14(3): 295-307. doi: 10.1016/j.jpha.2023.07.004

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Chemical derivatization strategies for enhancing the HPLC analytical performance of natural active triterpenoids

doi: 10.1016/j.jpha.2023.07.004

Xiao-Feng Huang^a,d,
Ying Xue^a,c,
Li Yong^c,
Tian-Tian Wang^a,b,
Pei Luo^{b
,
,},
Lin-Sen Qing^{a
,
,}

a. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China;
b. State Key Laboratory for Quality Research in Chinese Medicines, Macau University of Science and Technology, Macao, China;
c. Sichuan Center for Disease Control and Prevention, Chengdu, 610041, China;
d. University of Chinese Academy of Sciences, Beijing, 100049, China

Funds:

This work was financially supported by Sichuan Science and Technology Program (Grant No.: 2022ZYD0026), Biological Resources Program, Chinese Academy of Sciences (Grant No.: KFJ-BRP-008-007) and the Macao Science and Technology Development Fund (Grant No.: 0028/2019/AGJ).

Received Date: Apr. 10, 2023
Accepted Date: Jul. 06, 2023
Rev Recd Date: Jul. 06, 2023
Publish Date: Jul. 08, 2023

Abstract

Abstract

Triterpenoids widely exist in nature, displaying a variety of pharmacological activities. Determining triterpenoids in different matrices, especially in biological samples holds great significance. High-performance liquid chromatography (HPLC) has become the predominant method for triterpenoids analysis due to its exceptional analytical performance. However, due to the structural similarities among botanical samples, achieving effective separation of each triterpenoid proves challenging, necessitating significant improvements in analytical methods. Additionally, triterpenoids are characterized by a lack of ultraviolet (UV) absorption groups and chromophores, along with low ionization efficiency in mass spectrometry. Consequently, routine HPLC analysis suffers from poor sensitivity. Chemical derivatization emerges as an indispensable technique in HPLC analysis to enhance its performance. Considering the structural characteristics of triterpenoids, various derivatization reagents such as acid chlorides, rhodamines, isocyanates, sulfonic esters, and amines have been employed for the derivatization analysis of triterpenoids. This review comprehensively summarized the research progress made in derivatization strategies for HPLC detection of triterpenoids. Moreover, the limitations and challenges encountered in previous studies are discussed, and future research directions are proposed to develop more effective derivatization methods.
- Triterpenoid,
- HPLC,
- Derivatization,
- Quantification

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References(111)

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