Nylon 6-cellulose composite hosted in a hypodermic needle: Biofluid extraction and analysis by ambient mass spectrometry in a single device

Jaime Millán-Santiago; Rafael Lucena; Soledad Cárdenas

doi:10.1016/j.jpha.2023.06.015

Volume 13 Issue 11

Nov. 2023

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Article Navigation > Journal of Pharmaceutical Analysis > 2023 > 13(11): 1346-1352

Jaime Millán-Santiago, Rafael Lucena, Soledad Cárdenas. Nylon 6-cellulose composite hosted in a hypodermic needle: Biofluid extraction and analysis by ambient mass spectrometry in a single device[J]. Journal of Pharmaceutical Analysis, 2023, 13(11): 1346-1352. doi: 10.1016/j.jpha.2023.06.015

Citation:

Jaime Millán-Santiago, Rafael Lucena, Soledad Cárdenas. Nylon 6-cellulose composite hosted in a hypodermic needle: Biofluid extraction and analysis by ambient mass spectrometry in a single device[J]. Journal of Pharmaceutical Analysis, 2023, 13(11): 1346-1352. doi: 10.1016/j.jpha.2023.06.015

Citation:

Jaime Millán-Santiago, Rafael Lucena, Soledad Cárdenas. Nylon 6-cellulose composite hosted in a hypodermic needle: Biofluid extraction and analysis by ambient mass spectrometry in a single device[J]. Journal of Pharmaceutical Analysis, 2023, 13(11): 1346-1352. doi: 10.1016/j.jpha.2023.06.015

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Nylon 6-cellulose composite hosted in a hypodermic needle: Biofluid extraction and analysis by ambient mass spectrometry in a single device

doi: 10.1016/j.jpha.2023.06.015

Affordable and Sustainable Sample Preparation (AS2P) Research Group, Analytical Chemistry Department, Chemical Institute for Energy and Environment (IQUEMA), University of Cordoba, E-14071, Cordoba, Spain

Funds:

The grant “Biopolymer substrates for the determination of opioids in biofluids by ambient mass spectrometry” (Grant No.: PID2020-112862RB-I00) funded by MCIN/AEI/10.13039/501100011033 (Feder “Una manera de hacer Europa”) is gratefully acknowledged.

Received Date: Jan. 27, 2023
Accepted Date: Jun. 27, 2023
Rev Recd Date: May 28, 2023
Publish Date: Jun. 29, 2023

Abstract

Abstract

This study proposes a hypodermic needle (HN) as a sorbent holder and an electrospray (ESI) emitter, thus combining extraction and analysis in a single device. A novel nylon 6-cellulose (N6-Cel) composite sorbent is proposed to extract methadone from oral fluid samples. The cellulosic substrate provides the composite with high porosity, permitting the flow-through of the sample, while the polyamide contributes to the extraction of the analyte. The low price of the devices (considering the holder and the sorbent) contributes to the affordability of the method, and their small size allows easy transportation, opening the door to on-site extractions. Under the optimum conditions, the analyte can be determined by high-resolution ambient ionization mass spectrometry at a limit of detection (LOD) as low as 0.3 μg/L and precision (expressed as relative standard deviation, RSD) better than 9.3%. The trueness, expressed as relative recovery (RR), ranged from 90% to 109%. As high-resolution mass spectrometers are not available in many laboratories, the method was also adapted to low-resolution spectrometers. In this sense, the direct infusion of the eluates in a triple quadrupole-mass spectrometry provided an LOD of 2.2 μg/L. The RSD was better than 5.3%, and the RR ranged from 96% to 121%.
- ESI emitter,
- Mass spectrometry,
- Hypodermic needle,
- Composite,
- Methadone

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References(48)

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