Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics

Xiaoxiao Luo; Qirui Bi; Dongdong Huang; Yun Li; Changliang Yao; Jianqing Zhang; Wenlong Wei; Jiayuan Li; Zhenwei Li; Jingxian Zhang; Shen Ji; Yurong Wang; De-an Guo

doi:10.1016/j.jpha.2023.06.006

Volume 13 Issue 9

Sep. 2023

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Article Navigation > Journal of Pharmaceutical Analysis > 2023 > 13(9): 1070-1079

Xiaoxiao Luo, Qirui Bi, Dongdong Huang, Yun Li, Changliang Yao, Jianqing Zhang, Wenlong Wei, Jiayuan Li, Zhenwei Li, Jingxian Zhang, Shen Ji, Yurong Wang, De-an Guo. Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics[J]. Journal of Pharmaceutical Analysis, 2023, 13(9): 1070-1079. doi: 10.1016/j.jpha.2023.06.006

Citation:

Xiaoxiao Luo, Qirui Bi, Dongdong Huang, Yun Li, Changliang Yao, Jianqing Zhang, Wenlong Wei, Jiayuan Li, Zhenwei Li, Jingxian Zhang, Shen Ji, Yurong Wang, De-an Guo. Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics[J]. Journal of Pharmaceutical Analysis, 2023, 13(9): 1070-1079. doi: 10.1016/j.jpha.2023.06.006

Citation:

Xiaoxiao Luo, Qirui Bi, Dongdong Huang, Yun Li, Changliang Yao, Jianqing Zhang, Wenlong Wei, Jiayuan Li, Zhenwei Li, Jingxian Zhang, Shen Ji, Yurong Wang, De-an Guo. Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics[J]. Journal of Pharmaceutical Analysis, 2023, 13(9): 1070-1079. doi: 10.1016/j.jpha.2023.06.006

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Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics

doi: 10.1016/j.jpha.2023.06.006

a. School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, 211198, China;
b. Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China;
c. University of Chinese Academy of Sciences, Beijing, 100049, China;
d. NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China

Funds:

This work was financially supported by the Key Program of the National Natural Science Foundation of China (Grant No.: 82130111)

and the SIMM-SHUTCM Traditional Chinese Medicine Innovation Joint Research Program, China (Grant No.: E2G809H).

the Qi-Huang Chief Scientist Project of the National Administration of Traditional Chinese Medicine, China (2020)

the National Natural Science Foundation of China (Grant No.: 81803716)

Received Date: Mar. 16, 2023
Accepted Date: Jun. 13, 2023
Rev Recd Date: Jun. 10, 2023
Publish Date: Jun. 15, 2023

Abstract

Abstract

Pheretima, also called “earthworms”, is a well-known animal-derived traditional Chinese medicine that is extensively used in over 50 Chinese patent medicines (CPMs) in Chinese Pharmacopoeia (2020 edition). However, its zoological origin is unclear, both in the herbal market and CPMs. In this study, a strategy for integrating in-house annotated protein databases constructed from close evolutionary relationship-sourced RNA sequencing data from public archival resources and various sequencing algorithms (restricted search, open search, and de novo) was developed to characterize the phenotype of natural peptides of three major commercial species of Pheretima, including Pheretima aspergillum (PA), Pheretima vulgaris (PV), and Metaphire magna (MM). We identified 10,477 natural peptides in the PA, 7,451 in PV, and 5,896 in MM samples. Five specific signature peptides were screened and then validated using synthetic peptides; these demonstrated robust specificity for the authentication of PA, PV, and MM. Finally, all marker peptides were successfully applied to identify the zoological origins of Brain Heart capsules and Xiaohuoluo pills, revealing the inconsistent Pheretima species used in these CPMs. In conclusion, our integrated strategy could be used for the in-depth characterization of natural peptides of other animal-derived traditional Chinese medicines, especially non-model species with poorly annotated protein databases.
- Pheretima,
- Natural peptides,
- Label-free peptidomics,
- Proteogenomics,
- Animal derived medicine

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References(38)

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