Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition

Lei Gu; Xumiao Li; Wencheng Zhu; Yi Shen; Qinqin Wang; Wenjun Liu; Junfeng Zhang; Huiping Zhang; Jingquan Li; Ziyi Li; Zhen Liu; Chen Li; Hui Wang

doi:10.1016/j.jpha.2023.05.003

Volume 13 Issue 8

Aug. 2023

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Article Navigation > Journal of Pharmaceutical Analysis > 2023 > 13(8): 942-954

Lei Gu, Xumiao Li, Wencheng Zhu, Yi Shen, Qinqin Wang, Wenjun Liu, Junfeng Zhang, Huiping Zhang, Jingquan Li, Ziyi Li, Zhen Liu, Chen Li, Hui Wang. Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition[J]. Journal of Pharmaceutical Analysis, 2023, 13(8): 942-954. doi: 10.1016/j.jpha.2023.05.003

Citation:

Lei Gu, Xumiao Li, Wencheng Zhu, Yi Shen, Qinqin Wang, Wenjun Liu, Junfeng Zhang, Huiping Zhang, Jingquan Li, Ziyi Li, Zhen Liu, Chen Li, Hui Wang. Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition[J]. Journal of Pharmaceutical Analysis, 2023, 13(8): 942-954. doi: 10.1016/j.jpha.2023.05.003

Citation:

Lei Gu, Xumiao Li, Wencheng Zhu, Yi Shen, Qinqin Wang, Wenjun Liu, Junfeng Zhang, Huiping Zhang, Jingquan Li, Ziyi Li, Zhen Liu, Chen Li, Hui Wang. Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition[J]. Journal of Pharmaceutical Analysis, 2023, 13(8): 942-954. doi: 10.1016/j.jpha.2023.05.003

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Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition

doi: 10.1016/j.jpha.2023.05.003

Lei Gu^a,
Xumiao Li^a,
Wencheng Zhu^b,c,
Yi Shen^d,
Qinqin Wang^a,
Wenjun Liu^b,e,
Junfeng Zhang^d,
Huiping Zhang^d,
Jingquan Li^a,
Ziyi Li^{d
,
,},
Zhen Liu^{b,c
,
,},
Chen Li^{a
,
,},
Hui Wang^{a
,
,}

a. Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China;
b. Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, 200031, China;
c. Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 200031, China;
d. Shanghai Applied Protein Technology Co., Ltd., Shanghai, 201100, China;
e. University of Chinese Academy of Sciences, Beijing, 101408, China

Funds:

We thank all members of our laboratories for helpful discussions. This work was supported by the National Natural Science Foundation of China (NSFC) (Grant Nos.: 82030099 and 30700397), the National Key R&D Program of China (Grant No.: 2022YFD2101500), the Science and Technology Commission of Shanghai Municipality, China (Grant No.: 22DZ2303000), the Shanghai Municipal Science and Technology Commission “Science and Technology Innovation Action Plan” Technical Standard Project, China (Grant No.: 21DZ2201700), the Shanghai Municipal Science and Technology Commission “Science and Technology Innovation Action Plan” Natural Science Foundation Project, China (Grant No.: 23ZR1435800), the Strategic Priority Research Program of the Chinese Academy of Sciences, China (Grant No.: XDB32060000), the Basic Frontier Scientific Research Program of Chinese Academy of Sciences (Grant No.: ZDBS-LY-SM019), the Yangfan Project of Shanghai Science and Technology Commission, China (Grant No.: 22YF1454100), and the Innovative Research Team of High-level Local Universities in Shanghai, China.

Received Date: Dec. 29, 2022
Rev Recd Date: May 05, 2023

Abstract

Abstract

Single-cell or low-input multi-omics techniques have revolutionized the study of pre-implantation embryo development. However, the single-cell or low-input proteomic research in this field is relatively underdeveloped because of the higher threshold of the starting material for mammalian embryo samples and the lack of hypersensitive proteome technology. In this study, a comprehensive solution of ultrasensitive proteome technology (CS-UPT) was developed for single-cell or low-input mouse oocyte/embryo samples. The deep coverage and high-throughput routes significantly reduced the starting material and were selected by investigators based on their demands. Using the deep coverage route, we provided the first large-scale snapshot of the very early stage of mouse maternal-to-zygotic transition, including almost 5,500 protein groups from 20 mouse oocytes or zygotes for each sample. Moreover, significant protein regulatory networks centered on transcription factors and kinases between the MII oocyte and 1-cell embryo provided rich insights into minor zygotic genome activation.
- Single-cell proteomics,
- Low-input proteomics,
- Maternal-to-zygotic transition,
- Oocyte,
- Embryo

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References(94)

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