<i>Cux1</i><sup>+</sup> proliferative basal cells promote epidermal hyperplasia in chronic dry skin disease identified by single-cell RNA transcriptomics

Minhua Huang; Ning Hua; Siyi Zhuang; Qiuyuan Fang; Jiangming Shang; Zhen Wang; Xiaohua Tao; Jianguo Niu; Xiangyao Li; Peilin Yu; Wei Yang

doi:10.1016/j.jpha.2023.04.004

Volume 13 Issue 7

Jul. 2023

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Article Navigation > Journal of Pharmaceutical Analysis > 2023 > 13(7): 745-759

Minhua Huang, Ning Hua, Siyi Zhuang, Qiuyuan Fang, Jiangming Shang, Zhen Wang, Xiaohua Tao, Jianguo Niu, Xiangyao Li, Peilin Yu, Wei Yang. Cux1+ proliferative basal cells promote epidermal hyperplasia in chronic dry skin disease identified by single-cell RNA transcriptomics[J]. Journal of Pharmaceutical Analysis, 2023, 13(7): 745-759. doi: 10.1016/j.jpha.2023.04.004

Citation:

Minhua Huang, Ning Hua, Siyi Zhuang, Qiuyuan Fang, Jiangming Shang, Zhen Wang, Xiaohua Tao, Jianguo Niu, Xiangyao Li, Peilin Yu, Wei Yang. Cux1⁺ proliferative basal cells promote epidermal hyperplasia in chronic dry skin disease identified by single-cell RNA transcriptomics[J]. Journal of Pharmaceutical Analysis, 2023, 13(7): 745-759. doi: 10.1016/j.jpha.2023.04.004

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Cux1⁺ proliferative basal cells promote epidermal hyperplasia in chronic dry skin disease identified by single-cell RNA transcriptomics

doi: 10.1016/j.jpha.2023.04.004

a. Department of Biophysics, and Department of Neurology of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China;
b. Laboratory Medicine Center, Allergy Center, Department of Transfusion Medicine, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, 310000, China;
c. Department of Dermatology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, 310000, China;
d. Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, 750000, China;
e. Department of Neurology of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China;
f. Department of Toxicology, and Department of Medical Oncology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China;
g. MOE Frontier Science Center for Brain Research and Brain-Machine Integration, Zhejiang University School of Medicine, Hangzhou, 310000, China

Funds:

32071102 to P. Yu), the National Major Special Project on New Drug Innovation of China (Grant No.: 2018ZX09711001-004-005), the key research and development program of Ningxia Hui Autonomous Region (Grant No.: 2019BFH02003), Fundamental Research Funds for the Central Universities of China (Grant No.: 2016QNA7002 to P. Yu), and Zhejiang Provincial Natural Science Foundation (Grant No.: LR16H090001 to W. Yang). We thank J. Chen and Q. Huang from the Core Facilities, Zhejiang University School of Medicine for their technical support.

The work was supported by Technological Innovation 2030-Major Projects of Brain Science and Brain-like Research (Grant No.: 2022ZD0206200), the Natural Science Foundation of China (Grant Nos.: 31872796 and 82030108 to W. Yang

Received Date: Dec. 08, 2022
Accepted Date: Apr. 06, 2023
Rev Recd Date: Apr. 04, 2023
Publish Date: Apr. 11, 2023

Abstract

Abstract

Pathological dry skin is a disturbing and intractable healthcare burden, characterized by epithelial hyperplasia and severe itch. Atopic dermatitis (AD) and psoriasis models with complications of dry skin have been studied using single-cell RNA sequencing (scRNA-seq). However, scRNA-seq analysis of the dry skin mouse model (acetone/ether/water (AEW)-treated model) is still lacking. Here, we used scRNA-seq and in situ hybridization to identify a novel proliferative basal cell (PBC) state that exclusively expresses transcription factor CUT-like homeobox 1 (Cux1). Further in vitro study demonstrated that Cux1 is vital for keratinocyte proliferation by regulating a series of cyclin-dependent kinases (CDKs) and cyclins. Clinically, Cux1⁺ PBCs were increased in patients with psoriasis, suggesting that Cux1⁺ PBCs play an important part in epidermal hyperplasia. This study presents a systematic knowledge of the transcriptomic changes in a chronic dry skin mouse model, as well as a potential therapeutic target against dry skin-related dermatoses.
- scRNA-seq,
- Dry skin,
- Proliferative basal cells

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References(63)

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