DNA barcoding in herbal medicine: Retrospective and prospective

Shilin Chen; Xianmei Yin; Jianping Han; Wei Sun; Hui Yao; Jingyuan Song; Xiwen Li

doi:10.1016/j.jpha.2023.03.008

Volume 13 Issue 5

May 2023

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Shilin Chen, Xianmei Yin, Jianping Han, Wei Sun, Hui Yao, Jingyuan Song, Xiwen Li. DNA barcoding in herbal medicine: Retrospective and prospective[J]. Journal of Pharmaceutical Analysis, 2023, 13(5): 431-441. doi: 10.1016/j.jpha.2023.03.008

Citation:

Shilin Chen, Xianmei Yin, Jianping Han, Wei Sun, Hui Yao, Jingyuan Song, Xiwen Li. DNA barcoding in herbal medicine: Retrospective and prospective[J]. Journal of Pharmaceutical Analysis, 2023, 13(5): 431-441. doi: 10.1016/j.jpha.2023.03.008

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DNA barcoding in herbal medicine: Retrospective and prospective

doi: 10.1016/j.jpha.2023.03.008

a. Institute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China;
b. Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China;
c. State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China;
d. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China

Funds:

The research was funded by the National Natural Science Foundation of China (Grant No.: U1812403-1) and the China Academy of Chinese Medical Sciences Innovation Fund (Grant No.: CI2021A03910).

Received Date: Nov. 09, 2022
Accepted Date: Mar. 25, 2023
Rev Recd Date: Mar. 07, 2023
Publish Date: Apr. 05, 2023

Abstract

Abstract

DNA barcoding has been widely used for herb identification in recent decades, enabling safety and innovation in the field of herbal medicine. In this article, we summarize recent progress in DNA barcoding for herbal medicine to provide ideas for the further development and application of this technology. Most importantly, the standard DNA barcode has been extended in two ways. First, while conventional DNA barcodes have been widely promoted for their versatility in the identification of fresh or well-preserved samples, super-barcodes based on plastid genomes have rapidly developed and have shown advantages in species identification at low taxonomic levels. Second, mini-barcodes are attractive because they perform better in cases of degraded DNA from herbal materials. In addition, some molecular techniques, such as high-throughput sequencing and isothermal amplification, are combined with DNA barcodes for species identification, which has expanded the applications of herb identification based on DNA barcoding and brought about the post-DNA-barcoding era. Furthermore, standard and high-species coverage DNA barcode reference libraries have been constructed to provide reference sequences for species identification, which increases the accuracy and credibility of species discrimination based on DNA barcodes. In summary, DNA barcoding should play a key role in the quality control of traditional herbal medicine and in the international herb trade.
- Herbal identification,
- Super-barcode,
- Mini-barcode,
- Meta-barcoding,
- Cutting-edge barcoding

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References(155)

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