Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling

Lu Wang; Dandan Jiang; Lei Hua; Chuang Chen; Dongming Li; Weiguo Wang; Yiqian Xu; Qimu Yang; Haiyang Li; Song Leng

doi:10.1016/j.jpha.2023.02.008

Volume 13 Issue 4

Apr. 2023

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Lu Wang, Dandan Jiang, Lei Hua, Chuang Chen, Dongming Li, Weiguo Wang, Yiqian Xu, Qimu Yang, Haiyang Li, Song Leng. Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling[J]. Journal of Pharmaceutical Analysis, 2023, 13(4): 412-420. doi: 10.1016/j.jpha.2023.02.008

Citation:

Lu Wang, Dandan Jiang, Lei Hua, Chuang Chen, Dongming Li, Weiguo Wang, Yiqian Xu, Qimu Yang, Haiyang Li, Song Leng. Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling[J]. Journal of Pharmaceutical Analysis, 2023, 13(4): 412-420. doi: 10.1016/j.jpha.2023.02.008

Citation:

Lu Wang, Dandan Jiang, Lei Hua, Chuang Chen, Dongming Li, Weiguo Wang, Yiqian Xu, Qimu Yang, Haiyang Li, Song Leng. Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling[J]. Journal of Pharmaceutical Analysis, 2023, 13(4): 412-420. doi: 10.1016/j.jpha.2023.02.008

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Breath-by-breath measurement of exhaled ammonia by acetone-modifier positive photoionization ion mobility spectrometry via online dilution and purging sampling

doi: 10.1016/j.jpha.2023.02.008

Lu Wang^a,b,
Dandan Jiang^a,d,e,
Lei Hua^a,d,e,
Chuang Chen^a,d,e,
Dongming Li^a,d,e,
Weiguo Wang^a,d,e,
Yiqian Xu^a,c,d,e,
Qimu Yang^a,c,d,e,
Haiyang Li^{a,d,e
,
,},
Song Leng^{b
,
,}

a. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China;
b. Health Management Center, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, China;
c. University of Chinese Academy of Sciences, Beijing, 100049, China;
d. Dalian Key Laboratory for Online Analytical Instrumentation, Dalian, 116023, China;
e. Dalian Engineering Research Center of Breath Diagnostic Technology, Dalian, 116023, China

Funds:

This work was supported by the National Natural Science Foundation of China (Grant Nos.: 22027804, 21974141, and 21904125), Natural Science Foundation of Liaoning Province (Grant Nos.: 2022-MS-019 and 2022-MS-016), Science and Technology Innovation Foundation of Dalian (Grant No.: 2022JJ13SN096), Dalian Institute of Chemical Physics (Grant Nos.: DICP I202141 and DICP I202144), and 1 + X Program for Large Cohort Study-Clinical Research Incubation Project, The Second Hospital of Dalian Medical University (Project No.: 2022DXDL01).

Received Date: Oct. 04, 2022
Accepted Date: Feb. 16, 2023
Rev Recd Date: Feb. 14, 2023
Publish Date: Feb. 22, 2023

Abstract

Abstract

Exhaled ammonia (NH₃) is an essential noninvasive biomarker for disease diagnosis. In this study, an acetone-modifier positive photoionization ion mobility spectrometry (AM-PIMS) method was developed for accurate qualitative and quantitative analysis of exhaled NH₃ with high selectivity and sensitivity. Acetone was introduced into the drift tube along with the drift gas as a modifier, and the characteristic NH₃ product ion peak of (C₃H₆O)₄NH₄⁺ (K₀=1.45cm²/V·s) was obtained through the ion-molecule reaction with acetone reactant ions (C₃H₆O)₂H⁺ (K₀=1.87cm²/V·s), which significantly increased the peak-to-peak resolution and improved the accuracy of exhaled NH₃ qualitative identification. Moreover, the interference of high humidity and the memory effect of NH₃ molecules were significantly reduced via online dilution and purging sampling, thus realizing breath-by-breath measurement. As a result, a wide quantitative range of 5.87-140.92μmol/L with a response time of 40ms was achieved, and the exhaled NH₃ profile could be synchronized with the concentration curve of exhaled CO₂. Finally, the analytical capacity of AM-PIMS was demonstrated by measuring the exhaled NH₃ of healthy subjects, demonstrating its great potential for clinical disease diagnosis.
- Photoionization ion mobility spectrometry,
- Acetone modifier,
- Exhaled ammonia,
- Breath analysis

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References(41)

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