Drug adulteration analysis based on complexation with cyclodextrin and metal ions using ion mobility spectrometry

Zhigang Liang; Huanhuan Wang; Fangling Wu; Longfei Wang; Chenwei Li; Chuan-Fan Ding

doi:10.1016/j.jpha.2022.11.002

Volume 13 Issue 3

Mar. 2023

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Zhigang Liang, Huanhuan Wang, Fangling Wu, Longfei Wang, Chenwei Li, Chuan-Fan Ding. Drug adulteration analysis based on complexation with cyclodextrin and metal ions using ion mobility spectrometry[J]. Journal of Pharmaceutical Analysis, 2023, 13(3): 287-295. doi: 10.1016/j.jpha.2022.11.002

Citation:

Zhigang Liang, Huanhuan Wang, Fangling Wu, Longfei Wang, Chenwei Li, Chuan-Fan Ding. Drug adulteration analysis based on complexation with cyclodextrin and metal ions using ion mobility spectrometry[J]. Journal of Pharmaceutical Analysis, 2023, 13(3): 287-295. doi: 10.1016/j.jpha.2022.11.002

Citation:

Zhigang Liang, Huanhuan Wang, Fangling Wu, Longfei Wang, Chenwei Li, Chuan-Fan Ding. Drug adulteration analysis based on complexation with cyclodextrin and metal ions using ion mobility spectrometry[J]. Journal of Pharmaceutical Analysis, 2023, 13(3): 287-295. doi: 10.1016/j.jpha.2022.11.002

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Drug adulteration analysis based on complexation with cyclodextrin and metal ions using ion mobility spectrometry

doi: 10.1016/j.jpha.2022.11.002

a. Department of Thoracic Surgery, Ningbo First Hospital, Ningbo University, Ningbo, Zhejiang, 315010, China;
b. Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China

Funds:

This work was supported by the National Natural Science Foundation of China (Grant Nos.: 22004074 and 21927805), Zhejiang Natural Science Foundation (Grant No.: LY22B050006), and Foundation of Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry Technology and Molecular Detection (Grant No.: AMSMAKF2102).

Received Date: Jun. 17, 2022
Accepted Date: Nov. 02, 2022
Rev Recd Date: Nov. 02, 2022
Publish Date: Nov. 09, 2022

Abstract

Abstract

Drug adulteration and contamination are serious threats to human health therefore, their accurate monitoring is very important. Allopurinol (Alp) and theophylline (Thp) are commonly used drugs for the treatment of gout and bronchitis, while their isomers hypoxanthine (Hyt) and theobromine (Thm) have no effect and affect the efficacy of the drug. In this work, the drug isomers of Alp/Hyt and Thp/Thm are simply mixed with α-, β-, γ-cyclodextrin (CD) and metal ions and separated using trapped ion mobility spectrometry-mass spectrometry (TIMS-MS). TIMS-MS results showed that Alp/Hyt and Thp/Thm isomers could interact with CD and metal ions and form corresponding binary or ternary complexes to achieve their TIMS separation. Different metal ions and CDs showed different separation effect for the isomers, among which Alp and Hyt could be successfully distinguished from the complexes of [Alp/Hyt+γ-CD + Cu–H]⁺ with separation resolution (R_P–P) of 1.51; whereas Thp and Thm could be baseline separated by [Thp/Thm+γ-CD + Ca–H]⁺ with R_P–P of 1.96. Besides, chemical calculations revealed that the complexes were in the inclusion forms, and microscopic interactions were somewhat different, making their mobility separation. Moreover, relative and absolute quantification was investigated with an internal standard to determine the precise isomers content, and good linearity (R² > 0.99) was obtained. Finally, the method was applied for the adulteration detection where different drugs and urine were analyzed. In addition, due to the advantages of fast speed, simple operation, high sensitivity, and no chromatographic separation required, the proposed method provides an effective strategy for the drug adulteration detection of isomers.
- Drug isomer,
- Adulteration,
- Separation,
- Ion mobility,
- Chemical calculations

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References(41)

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