A benzenesulfonic acid-modified organic polymer monolithic column with reversed-phase/hydrophilic bifunctional selectivity for capillary electrochromatography

Yikun Liu; Ning He; Yingfang Lu; Weiqiang Li; Xin He; Zhentao Li; Zilin Chen

doi:10.1016/j.jpha.2022.10.006

Volume 13 Issue 2

Mar. 2023

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Article Navigation > Journal of Pharmaceutical Analysis > 2023 > 13(2): 209-215

Yikun Liu, Ning He, Yingfang Lu, Weiqiang Li, Xin He, Zhentao Li, Zilin Chen. A benzenesulfonic acid-modified organic polymer monolithic column with reversed-phase/hydrophilic bifunctional selectivity for capillary electrochromatography[J]. Journal of Pharmaceutical Analysis, 2023, 13(2): 209-215. doi: 10.1016/j.jpha.2022.10.006

Citation:

Yikun Liu, Ning He, Yingfang Lu, Weiqiang Li, Xin He, Zhentao Li, Zilin Chen. A benzenesulfonic acid-modified organic polymer monolithic column with reversed-phase/hydrophilic bifunctional selectivity for capillary electrochromatography[J]. Journal of Pharmaceutical Analysis, 2023, 13(2): 209-215. doi: 10.1016/j.jpha.2022.10.006

Citation:

Yikun Liu, Ning He, Yingfang Lu, Weiqiang Li, Xin He, Zhentao Li, Zilin Chen. A benzenesulfonic acid-modified organic polymer monolithic column with reversed-phase/hydrophilic bifunctional selectivity for capillary electrochromatography[J]. Journal of Pharmaceutical Analysis, 2023, 13(2): 209-215. doi: 10.1016/j.jpha.2022.10.006

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A benzenesulfonic acid-modified organic polymer monolithic column with reversed-phase/hydrophilic bifunctional selectivity for capillary electrochromatography

doi: 10.1016/j.jpha.2022.10.006

Yikun Liu^a,b,
Ning He^a,b,
Yingfang Lu^a,
Weiqiang Li^a,
Xin He^a,
Zhentao Li^a,b,
Zilin Chen^{a,b
,
,}

a. Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430072, China;
b. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, Wuhan, 430071, China

Funds:

This work was supported by the National Natural Science Foundation of China (Grant Nos.: 82273885, 82073808 and 81872828).

Received Date: May 20, 2022
Accepted Date: Oct. 31, 2022
Rev Recd Date: Oct. 30, 2022
Publish Date: Mar. 07, 2023

Abstract

Abstract

Here, a styrene-based polymer monolithic column poly(VBS-co-TAT-co-AHM) with reversed-phase/hydrophilic interaction liquid chromatography (RPLC/HILIC) bifunctional separation mode was successfully prepared for capillary electrochromatography by the in situ polymerization of sodium p-styrene sulfonate (VBS) with cross-linkers 3-(acryloyloxy)-2-hydroxypropyl methacrylate (AHM) and 1,3,5-triacryloylhexahydro-1,3,5-triazine (TAT). The preparation conditions of the monolith were optimized. The morphology and formation of the poly(VBS-co-TAT-co-AHM) monolith were confirmed by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). The separation performances of the monolith were evaluated systematically. It should be noted that the incorporation of VBS functional monomer can provide π-π interactions, hydrophilic interactions, and ion-exchange interactions. Hence, the prepared poly(VBS-co-TAT-co-AHM) monolith can achieve efficient separation of thiourea compounds, benzene series, phenol compounds, aniline compounds and sulfonamides in RPLC or HILIC separation mode. The largest theoretical plate number for N,N'-dimethylthiourea reached 1.7×10⁵ plates/m. In addition, the poly(VBS-co-TAT-co-AHM) monolithic column showed excellent reproducibility and stability. This novel monolithic column has great application value and potential in capillary electrochromatography (CEC).
- Sodium p-styrene sulfona,
- Stationary phase,
- Monolithic column,
- Capillary electrochromatography,
- Bifunctional interaction

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References(33)

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