Comparison of different approaches for direct coupling of solid-phase microextraction to mass spectrometry for drugs of abuse analysis in plasma

Wei Zhou; Martyna N. Wieczorek; Runshan Will Jiang; Janusz Pawliszyn

doi:10.1016/j.jpha.2022.10.004

Volume 13 Issue 2

Mar. 2023

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Wei Zhou, Martyna N. Wieczorek, Runshan Will Jiang, Janusz Pawliszyn. Comparison of different approaches for direct coupling of solid-phase microextraction to mass spectrometry for drugs of abuse analysis in plasma[J]. Journal of Pharmaceutical Analysis, 2023, 13(2): 216-222. doi: 10.1016/j.jpha.2022.10.004

Citation:

Wei Zhou, Martyna N. Wieczorek, Runshan Will Jiang, Janusz Pawliszyn. Comparison of different approaches for direct coupling of solid-phase microextraction to mass spectrometry for drugs of abuse analysis in plasma[J]. Journal of Pharmaceutical Analysis, 2023, 13(2): 216-222. doi: 10.1016/j.jpha.2022.10.004

Citation:

Wei Zhou, Martyna N. Wieczorek, Runshan Will Jiang, Janusz Pawliszyn. Comparison of different approaches for direct coupling of solid-phase microextraction to mass spectrometry for drugs of abuse analysis in plasma[J]. Journal of Pharmaceutical Analysis, 2023, 13(2): 216-222. doi: 10.1016/j.jpha.2022.10.004

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Comparison of different approaches for direct coupling of solid-phase microextraction to mass spectrometry for drugs of abuse analysis in plasma

doi: 10.1016/j.jpha.2022.10.004

a. Department of Chemistry, University of Waterloo, Waterloo, ON, N2L 3G1, Canada;
b. Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Poznań, Poland

Funds:

We would like to thank Shimadzu Scientific Instruments (Columbia, MD, USA) and Shimadzu Corporation (Kyoto, Japan) for providing Shimadzu LCMS 8060 MS with a PESI ionization interface used in this work, as well as for their financial support. We would also like to thank Restek Corporation for providing the commercial CBS blades. Finally, we are grateful to the Natural Science and Engineering Research Council (NSERC) of Canada for their financial support through the Industrial Research Chair program. This study was also financially supported by the National Science Centre, Poland (Grant No.: 2020/04/X/NZ9/01281).

Received Date: Aug. 25, 2022
Accepted Date: Oct. 28, 2022
Rev Recd Date: Oct. 12, 2022
Publish Date: Mar. 07, 2023

Abstract

Abstract

The direct coupling of solid-phase microextraction (SPME) to mass spectrometry (MS) (SPME-MS) has proven to be an effective method for the fast screening and quantitative analysis of compounds in complex matrices such as blood and plasma. In recent years, our lab has developed three novel SPME-MS techniques: SPME-microfluidic open interface-MS (SPME-MOI-MS), coated blade spray-MS (CBS-MS), and SPME-probe electrospray ionization-MS (SPME-PESI-MS). The fast and high-throughput nature of these SPME-MS technologies makes them attractive options for point-of-care analysis and anti-doping testing. However, all these three techniques utilize different SPME geometries and were tested with different MS instruments. Lack of comparative data makes it difficult to determine which of these methodologies is the best option for any given application. This work fills this gap by making a comprehensive comparison of these three technologies with different SPME devices including SPME fibers, CBS blades, and SPME-PESI probes and SPME-liquid chromatography-MS (SPME-LC-MS) for the analysis of drugs of abuse using the same MS instrument. Furthermore, for the first time, we developed different desorption chambers for MOI-MS for coupling with SPME fibers, CBS blades, and SPME-PESI probes, thus illustrating the universality of this approach. In total, eight analytical methods were developed, with the experimental data showing that all the SPME-based methods provided good analytical performance with R² of linearities larger than 0.9925, accuracies between 81% and 118%, and good precision with an RSD%≤13%.
- Solid-phase microextraction,
- Mass spectrometry,
- Microfluidic open interface,
- Coated blade spray,
- Probe electrospray ionization,
- Drug of abuse

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References(26)

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